Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF)

Dysregulation of VEGF-A bioavailability has been implicated in the development of lung injury/fibrosis, exemplified by Idiopathic Pulmonary Fibrosis (IPF). VEGF-A is a target of the hypoxic response via its translational regulation by HIF-1α. The role of hypoxia and hyperoxia in the development and...

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Main Authors: Barratt, Shaney L., Blythe, Thomas, Ourradi, Khadija, Jarrett, Caroline, Welsh, Gavin I., Bates, David O., Millar, Ann B.
Format: Article
Published: BioMed Central 2018
Subjects:
Online Access:https://eprints.nottingham.ac.uk/50178/
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author Barratt, Shaney L.
Blythe, Thomas
Ourradi, Khadija
Jarrett, Caroline
Welsh, Gavin I.
Bates, David O.
Millar, Ann B.
author_facet Barratt, Shaney L.
Blythe, Thomas
Ourradi, Khadija
Jarrett, Caroline
Welsh, Gavin I.
Bates, David O.
Millar, Ann B.
author_sort Barratt, Shaney L.
building Nottingham Research Data Repository
collection Online Access
description Dysregulation of VEGF-A bioavailability has been implicated in the development of lung injury/fibrosis, exemplified by Idiopathic Pulmonary Fibrosis (IPF). VEGF-A is a target of the hypoxic response via its translational regulation by HIF-1α. The role of hypoxia and hyperoxia in the development and progression of IPF has not been explored. In normal lung (NF) and IPF-derived fibroblasts (FF) VEGF-Aa protein expression was upregulated by hypoxia, mediated through activation of VEGF-Aa gene transcription. VEGF-A receptors and co-receptors were differentially expressed by hypoxia and hyperoxia. Our data supports a potential role for hypoxia, hyperoxia and VEGF-Aa isoforms as drivers of fibrogenesis.
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spelling nottingham-501782020-05-04T19:26:43Z https://eprints.nottingham.ac.uk/50178/ Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF) Barratt, Shaney L. Blythe, Thomas Ourradi, Khadija Jarrett, Caroline Welsh, Gavin I. Bates, David O. Millar, Ann B. Dysregulation of VEGF-A bioavailability has been implicated in the development of lung injury/fibrosis, exemplified by Idiopathic Pulmonary Fibrosis (IPF). VEGF-A is a target of the hypoxic response via its translational regulation by HIF-1α. The role of hypoxia and hyperoxia in the development and progression of IPF has not been explored. In normal lung (NF) and IPF-derived fibroblasts (FF) VEGF-Aa protein expression was upregulated by hypoxia, mediated through activation of VEGF-Aa gene transcription. VEGF-A receptors and co-receptors were differentially expressed by hypoxia and hyperoxia. Our data supports a potential role for hypoxia, hyperoxia and VEGF-Aa isoforms as drivers of fibrogenesis. BioMed Central 2018-01-15 Article PeerReviewed Barratt, Shaney L., Blythe, Thomas, Ourradi, Khadija, Jarrett, Caroline, Welsh, Gavin I., Bates, David O. and Millar, Ann B. (2018) Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF). Respiratory Research, 19 . p. 9. ISSN 1465-9921 Interstitial lung disease Vascular endothelial growth factor Hypoxia Idiopathic pulmonary fibrosis https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-017-0711-x doi:10.1186/s12931-017-0711-x doi:10.1186/s12931-017-0711-x
spellingShingle Interstitial lung disease
Vascular endothelial growth factor
Hypoxia
Idiopathic pulmonary fibrosis
Barratt, Shaney L.
Blythe, Thomas
Ourradi, Khadija
Jarrett, Caroline
Welsh, Gavin I.
Bates, David O.
Millar, Ann B.
Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF)
title Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF)
title_full Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF)
title_fullStr Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF)
title_full_unstemmed Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF)
title_short Effects of hypoxia and hyperoxia on the differential expression of VEGF-A isoforms and receptors in Idiopathic Pulmonary Fibrosis (IPF)
title_sort effects of hypoxia and hyperoxia on the differential expression of vegf-a isoforms and receptors in idiopathic pulmonary fibrosis (ipf)
topic Interstitial lung disease
Vascular endothelial growth factor
Hypoxia
Idiopathic pulmonary fibrosis
url https://eprints.nottingham.ac.uk/50178/
https://eprints.nottingham.ac.uk/50178/
https://eprints.nottingham.ac.uk/50178/