Simplified footprint-free Cas9/CRISPR editing of cardiac-associated genes in human pluripotent stem cells

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Simplified footprint-free Cas9/CRISPR editing of cardiac-associated genes in human pluripotent stem cells

Modelling disease with hPSCs is hindered because the impact on cell phenotype from genetic variability between individuals can be greater than from the pathogenic mutation. While ‘footprint-free’ Cas9/CRISPR editing solves this issue, existing approaches are inefficient or lengthy. Here, a simplifie...

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Bibliographic Details
Main Authors:	Kondrashov, Alexander, Hoang, Minh Duc, Smith, James G.W., Bhagwan, Jamie R., Duncan, Gary, Mosqueira, Diogo, Barbadillo Munoz, Maria, Vo, Nguyen T.N., Denning, Chris
Format:	Article
Language:	English
Published:	Mary Ann Liebert 2018
Subjects:	Cas9/CRISPR; PiggyBac; gene editing; human pluripotent stem cells; genetic disease modelling; cardiomyocytes
Online Access:	https://eprints.nottingham.ac.uk/49543/

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