Virtual genome walking across the 32 Gb Ambystoma mexicanum genome; assembling gene models and intronic sequence

Large repeat rich genomes present challenges for assembly using short read technologies. The 32 Gb axolotl genome is estimated to contain ~19 Gb of repetitive DNA making an assembly from short reads alone effectively impossible. Indeed, this model species has been sequenced to 20× coverage but the r...

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Bibliographic Details
Main Authors: Evans, Teri, D. Johnson, Andrew, Loose, Matthew
Format: Article
Published: Nature Publishing Group 2018
Online Access:https://eprints.nottingham.ac.uk/49288/