A protein microarray assay for serological determination of antigen-specific antibody responses following Clostridium difficile infection

We provide a detailed overview of a novel high-throughput protein microarray assay for the determination of anti-C. difficile antibody levels in human sera and in separate preparations of polyclonal IVIg. The protocol describes the methodological steps involved in sample preparation, printing of arr...

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Bibliographic Details
Main Authors: Negm, Ola H., Hamed, Mohamed R., Monaghan, Tanya M.
Format: Article
Published: Journal of Visualized Experiments 2018
Subjects:
Online Access:https://eprints.nottingham.ac.uk/49192/