Analysis of DNA binding and translocation by the E. coli CRISPR immunity protein Cas3

QR Code

Analysis of DNA binding and translocation by the E. coli CRISPR immunity protein Cas3

Cas3 is essential for CRISPR-Cas immunity in Escherichia coli and is conserved across Class 1, type I CRISPR-Cas systems. Cas3 is a metal-dependent nuclease and ATP-dependent helicase that is required for CRISPR interference reactions, the stage of immunity in which a Cascade complex delivers crRNA...

Full description

Bibliographic Details
Main Author:	He, Liu
Format:	Thesis (University of Nottingham only)
Language:	English
Published:	2018
Online Access:	https://eprints.nottingham.ac.uk/49136/

Similar Items

Analysis of Cas1-2 and Cas3 CRISPR immunity proteins in E. coli cell physiology
by: Reeves, Ryan Peter
Published: (2019)

Different genome stability proteins underpin primed and naïve adaptation in E. coli CRISPR-Cas immunity
by: Ivančić-Baće, Ivana, et al.
Published: (2015)

Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS
by: Majsec, Kristina, et al.
Published: (2016)

Analysis of the E. coli CRISPR Cas3 protein and its potential for regulation by a short non-coding RNA
by: Kennerdale, Niamh
Published: (2024)

CRISPR-Cas immunity: analysis of adaptation and interference reactions in prokaryotes
by: Cass, S.D.B.
Published: (2016)

Characterising Host Factors Required for CRISPR Cas Adaptation in Escherichia coli
by: Edwards, Harry
Published: (2023)

Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3
by: Gong, Bei, et al.
Published: (2014)

CRISPR systems - molecular mechanisms of the interaction between Cas1 and DnaK
by: Stewart, Alison L.
Published: (2025)

Towards DNA‑free CRISPR/Cas9 genome editing for sustainable oil palm improvement
by: Shaharuddin, Noor Azmi, et al.
Published: (2024)

Investigation of CRISPR-Cas adaptation mechanism through exploration of Cas4-1 fusions
by: Walker, Emily
Published: (2019)

The role of Cas8 in type I CRISPR interference
by: Cass, Simon, et al.
Published: (2015)

Part A: Application of fluorescent markers onto CRISPR-associated proteins/ Part B: Substitution of cysteine residues in CRISPR associated Protein Cas1
by: Parr, Lochlan
Published: (2024)

CRISPR-Cas Adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonised by 5’ ssDNA exonucleases
by: Killelea, Tom, et al.
Published: (2018)

Campylobacter jejuni acquire new host-derived CRISPR spacers when in association with bacteriophages harboring a CRISPR-like Cas4 protein
by: Hooton, Steven P.T., et al.
Published: (2015)

Harnessing CRISPR-Cas to combat COVID-19: from diagnostics to therapeutics
by: Kok, Gan Chan, et al.
Published: (2021)

CRISPR-Cas knockout of miR21 reduces glioma growth
by: Nieland, Lisa, et al.
Published: (2022)

The enhancement of plant disease resistance using CRISPR/Cas9 technology
by: Mohd Azman, Amier Asyraf, et al.
Published: (2022)

Applications of a high-throughput screen detecting CRISPR-Cas spacer acquisition
by: Cannon, Christopher
Published: (2025)

Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro
by: Ravichantar, Nithya
Published: (2020)

DNA-free CRISPR/Cas9 genome editing system for oil palm protoplasts using multiple ribonucleoproteins (RNPs) complexes
by: Norfaezah, Jamaludin, et al.
Published: (2024)

Gene editing the phytoene desaturase alleles of Cavendish banana using CRISPR/Cas9
by: Eftekhari, Fatima, et al.
Published: (2018)

Precision Genome Engineering and Gene Silencing Using CRISPR/dCas9 System
by: Arooj, Mahira
Published: (2018)

Development of CRISPR-cas9 ribonucleoprotein complex platform for disruption of candidalysin in Candida albicans
by: Ahmad, Hasna, et al.
Published: (2020)

The expansion of available bioparts and the development of CRISPR/Cas9 for genetic engineering of Parageobacillus thermoglucosidasius
by: Lau, Matthew Simon Harold
Published: (2019)

CRISPR/dCas9 platforms in plants: strategies and applications beyond genome editing
by: Moradpour, Mahdi, et al.
Published: (2019)

CRISPR-Cas9-mediated genome editing of the extracellular matrix gene TNC in human fibroblasts
by: Brunetti, Giulio
Published: (2017)

A refined method to study gene dosage changes in-vitro using CRISPR/Cas9
by: Raposo, T.P., et al.
Published: (2017)

The current landscape of CRISPR-Cas9-based technologies for imaging of genomic loci in fixed and live cells
by: Yu, Choo Yee, et al.
Published: (2024)

Elucidating the molecular mechanism of drug binding and translocation by the multidrug transporter ABCG2
by: Kapoor, Parth
Published: (2020)

Simplified footprint-free Cas9/CRISPR editing of cardiac-associated genes in human pluripotent stem cells
by: Kondrashov, Alexander, et al.
Published: (2018)

Development of CRISPR-Cas9-mediated site-directed mutagenesis of ECE1 gene (candidalysin) in Candida albicans
by: Engku Nasrullah Satiman, Engku Anis Fariha, et al.
Published: (2020)

Waking up dormant tumor suppressor genes with zinc fingers, TALEs and the CRISPR/dCas9 system
by: Garcia-Bloj, B., et al.
Published: (2016)

Harnessing CRISPR/Cas9 system to engineer disease resistance in solanaceous crops: current progress and future prospects
by: Ahmed, Shiuli, et al.
Published: (2025)

Structural evidence that colicin a protein binds to a novel binding site of TolA protein in Escherichia coli periplasm
by: Li, Chan, et al.
Published: (2012)

Genetic analysis of RPA single-stranded DNA binding protein in Haloferax volcanii
by: Stroud, A. L.
Published: (2012)

Correction Of Glucose-6-Phosphate Dehydrogenase (G6PD) Viangchan Mutation In Monocytes Using CRISPR/CAS9
by: Vengidasan, Lelamekala
Published: (2018)

Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs
by: Bertero, Alessandro, et al.
Published: (2016)

Translocating under the radar: an investigation into the secretion pathway of EspC, a ‘classical’ autotransporter from enteropathogenic E. coli
by: Serdar, Marina
Published: (2023)

Functional interrogation of CRISPR-CAS9 mediated EpCAM knockout in breast cancer cells / Afiqah Fasihah Abdul Rahim
by: Afiqah Fasihah , Abdul Rahim
Published: (2022)

Sequence-specific inhibition of microRNA-130a gene by CRISPR/Cas9 system in breast cancer cell line
by: Abdollah, Nur Ainina, et al.
Published: (2017)