Eucheuma cottonii sulfated oligosaccharides decrease food allergic responses in animal models by up-regulating regulatory T (Treg) cells
In the present study, the anti-food allergy activity of Eucheuma cottonii sulfated oligosaccharide (ESO) was investigated. ESO was obtained by enzymatic degradation and purified by column chromatography. RBL-2H3 cells and BALB/c mouse model were used to test the anti-food allergy activity of ESO. Th...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
American Chemical Society
2017
|
| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/45461/ |
| _version_ | 1848797135823175680 |
|---|---|
| author | Xu, Sha-Sha Liu, Qing-Mei Xiao, An-Feng Maleki, Soheila J. Alcocer, Marcos Gao, Yuan-Yuan Cao, Min-Jie Liu, Guang-Ming |
| author_facet | Xu, Sha-Sha Liu, Qing-Mei Xiao, An-Feng Maleki, Soheila J. Alcocer, Marcos Gao, Yuan-Yuan Cao, Min-Jie Liu, Guang-Ming |
| author_sort | Xu, Sha-Sha |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | In the present study, the anti-food allergy activity of Eucheuma cottonii sulfated oligosaccharide (ESO) was investigated. ESO was obtained by enzymatic degradation and purified by column chromatography. RBL-2H3 cells and BALB/c mouse model were used to test the anti-food allergy activity of ESO. The effects of ESO on the regulatory T (Treg) cells and bone marrow-derived mast cells (BMMCs) were investigated by flow cytometry. The results of in vivo assay showed that ESO decreased the levels of mast cell protease-1 and histamine and inhibited the levels of specific IgE by 77.7%. In addition, the production of interleukin (IL)-4 and IL-13 was diminished in the ESO groups compared to the non-ESO-treated group. Furthermore, ESO could up-regulate Treg cells by 22.2−97.1%. In conclusion, ESO decreased the allergy response in mice by reducing basophil degranulation, up-regulating Treg cells via Forkhead box protein 3 (Foxp3), and releasing IL-10. ESO may have preventive and therapeutic potential in allergic disease. |
| first_indexed | 2025-11-14T19:59:04Z |
| format | Article |
| id | nottingham-45461 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:59:04Z |
| publishDate | 2017 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-454612020-05-04T18:39:58Z https://eprints.nottingham.ac.uk/45461/ Eucheuma cottonii sulfated oligosaccharides decrease food allergic responses in animal models by up-regulating regulatory T (Treg) cells Xu, Sha-Sha Liu, Qing-Mei Xiao, An-Feng Maleki, Soheila J. Alcocer, Marcos Gao, Yuan-Yuan Cao, Min-Jie Liu, Guang-Ming In the present study, the anti-food allergy activity of Eucheuma cottonii sulfated oligosaccharide (ESO) was investigated. ESO was obtained by enzymatic degradation and purified by column chromatography. RBL-2H3 cells and BALB/c mouse model were used to test the anti-food allergy activity of ESO. The effects of ESO on the regulatory T (Treg) cells and bone marrow-derived mast cells (BMMCs) were investigated by flow cytometry. The results of in vivo assay showed that ESO decreased the levels of mast cell protease-1 and histamine and inhibited the levels of specific IgE by 77.7%. In addition, the production of interleukin (IL)-4 and IL-13 was diminished in the ESO groups compared to the non-ESO-treated group. Furthermore, ESO could up-regulate Treg cells by 22.2−97.1%. In conclusion, ESO decreased the allergy response in mice by reducing basophil degranulation, up-regulating Treg cells via Forkhead box protein 3 (Foxp3), and releasing IL-10. ESO may have preventive and therapeutic potential in allergic disease. American Chemical Society 2017-03-30 Article PeerReviewed Xu, Sha-Sha, Liu, Qing-Mei, Xiao, An-Feng, Maleki, Soheila J., Alcocer, Marcos, Gao, Yuan-Yuan, Cao, Min-Jie and Liu, Guang-Ming (2017) Eucheuma cottonii sulfated oligosaccharides decrease food allergic responses in animal models by up-regulating regulatory T (Treg) cells. Journal of Agricultral and Food Chemistry, 65 (15). pp. 3212-3222. ISSN 1520-5118 Eucheuma cottonii sulfated oligosaccharide (ESO); food allergy; mast cells; murine model; Treg cells http://pubs.acs.org/doi/abs/10.1021/acs.jafc.7b00389 doi:10.1021/acs.jafc.7b00389 doi:10.1021/acs.jafc.7b00389 |
| spellingShingle | Eucheuma cottonii sulfated oligosaccharide (ESO); food allergy; mast cells; murine model; Treg cells Xu, Sha-Sha Liu, Qing-Mei Xiao, An-Feng Maleki, Soheila J. Alcocer, Marcos Gao, Yuan-Yuan Cao, Min-Jie Liu, Guang-Ming Eucheuma cottonii sulfated oligosaccharides decrease food allergic responses in animal models by up-regulating regulatory T (Treg) cells |
| title | Eucheuma cottonii sulfated oligosaccharides decrease food
allergic responses in animal models by up-regulating regulatory T (Treg) cells |
| title_full | Eucheuma cottonii sulfated oligosaccharides decrease food
allergic responses in animal models by up-regulating regulatory T (Treg) cells |
| title_fullStr | Eucheuma cottonii sulfated oligosaccharides decrease food
allergic responses in animal models by up-regulating regulatory T (Treg) cells |
| title_full_unstemmed | Eucheuma cottonii sulfated oligosaccharides decrease food
allergic responses in animal models by up-regulating regulatory T (Treg) cells |
| title_short | Eucheuma cottonii sulfated oligosaccharides decrease food
allergic responses in animal models by up-regulating regulatory T (Treg) cells |
| title_sort | eucheuma cottonii sulfated oligosaccharides decrease food
allergic responses in animal models by up-regulating regulatory t (treg) cells |
| topic | Eucheuma cottonii sulfated oligosaccharide (ESO); food allergy; mast cells; murine model; Treg cells |
| url | https://eprints.nottingham.ac.uk/45461/ https://eprints.nottingham.ac.uk/45461/ https://eprints.nottingham.ac.uk/45461/ |