A refined method to study gene dosage changes in-vitro using CRISPR/Cas9

Aims: Gene dosage can have a major impact on cell biology although, hitherto, is has been difficult to study using in-vitro models. We sought to refine and accelerate the development of “gene dosage” models through using CRISPR/Cas9 (a gene-editing technology) for sequential knockout of gene alleles...

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Bibliographic Details
Main Authors: Raposo, T.P., Ebili, Henry, Ilyas, Mohammad
Format: Article
Published: BMJ Publishing Group Ltd 2017
Online Access:https://eprints.nottingham.ac.uk/44871/