Dually sensitive dextran-based micelles for methotrexate delivery
Temperature-sensitive polymeric micelles were prepared from dextran grafted with poly(N-isopropylacrylamide) (PNIPAAm) or polyethylene glycol methyl ether (PEGMA) via controlled radical polymerization and evaluated as delivery systems of the anticancer drug methotrexate (MTX). Polymer-grafting was c...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Published: |
Royal Society of Chemistry
2017
|
| Online Access: | https://eprints.nottingham.ac.uk/44129/ |
| _version_ | 1848796845326729216 |
|---|---|
| author | Blanco-Fernandez, B. Concheiro, A. Makwana, H. Fernandez-Trillo, Francisco Alexander, Cameron Alvarez-Lorenzo, C. |
| author_facet | Blanco-Fernandez, B. Concheiro, A. Makwana, H. Fernandez-Trillo, Francisco Alexander, Cameron Alvarez-Lorenzo, C. |
| author_sort | Blanco-Fernandez, B. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Temperature-sensitive polymeric micelles were prepared from dextran grafted with poly(N-isopropylacrylamide) (PNIPAAm) or polyethylene glycol methyl ether (PEGMA) via controlled radical polymerization and evaluated as delivery systems of the anticancer drug methotrexate (MTX). Polymer-grafting was carried out after introduction of initiating groups onto the polysaccharide backbone, without the need for protection of hydroxyl groups and avoiding the use of toxic solvents. Temperature-responsive dextran-based copolymers were designed to exhibit self-aggregation behaviour, affinity for MTX and high cellular internalization. In addition, some grafted polymers incorporated 2-aminoethyl methacrylate to reinforce MTX encapsulation in the micelles by means of ionic interactions. Dextran-based micelles were cytocompatible and had an appropriate size to be used as drug carriers. MTX release was dependent on the pH and temperature. The combination of poly(2-aminoethylmethacrylate) and PNIPAAm with the dextran backbone permitted the complete release of MTX at normal physiological temperature. Co-polymer micelles were highly internalized by tumour cells (CHO-K1) and, when loaded with MTX, led to enhanced cytotoxicity compared to the free drug. |
| first_indexed | 2025-11-14T19:54:27Z |
| format | Article |
| id | nottingham-44129 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:54:27Z |
| publishDate | 2017 |
| publisher | Royal Society of Chemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-441292020-05-04T18:36:51Z https://eprints.nottingham.ac.uk/44129/ Dually sensitive dextran-based micelles for methotrexate delivery Blanco-Fernandez, B. Concheiro, A. Makwana, H. Fernandez-Trillo, Francisco Alexander, Cameron Alvarez-Lorenzo, C. Temperature-sensitive polymeric micelles were prepared from dextran grafted with poly(N-isopropylacrylamide) (PNIPAAm) or polyethylene glycol methyl ether (PEGMA) via controlled radical polymerization and evaluated as delivery systems of the anticancer drug methotrexate (MTX). Polymer-grafting was carried out after introduction of initiating groups onto the polysaccharide backbone, without the need for protection of hydroxyl groups and avoiding the use of toxic solvents. Temperature-responsive dextran-based copolymers were designed to exhibit self-aggregation behaviour, affinity for MTX and high cellular internalization. In addition, some grafted polymers incorporated 2-aminoethyl methacrylate to reinforce MTX encapsulation in the micelles by means of ionic interactions. Dextran-based micelles were cytocompatible and had an appropriate size to be used as drug carriers. MTX release was dependent on the pH and temperature. The combination of poly(2-aminoethylmethacrylate) and PNIPAAm with the dextran backbone permitted the complete release of MTX at normal physiological temperature. Co-polymer micelles were highly internalized by tumour cells (CHO-K1) and, when loaded with MTX, led to enhanced cytotoxicity compared to the free drug. Royal Society of Chemistry 2017-03-06 Article PeerReviewed Blanco-Fernandez, B., Concheiro, A., Makwana, H., Fernandez-Trillo, Francisco, Alexander, Cameron and Alvarez-Lorenzo, C. (2017) Dually sensitive dextran-based micelles for methotrexate delivery. RSC Advances, 7 (24). pp. 14448-14460. ISSN 2046-2069 http://pubs.rsc.org/en/Content/ArticleLanding/2017/RA/C7RA00696A#!divAbstract doi:10.1039/c7ra00696a doi:10.1039/c7ra00696a |
| spellingShingle | Blanco-Fernandez, B. Concheiro, A. Makwana, H. Fernandez-Trillo, Francisco Alexander, Cameron Alvarez-Lorenzo, C. Dually sensitive dextran-based micelles for methotrexate delivery |
| title | Dually sensitive dextran-based micelles for methotrexate delivery |
| title_full | Dually sensitive dextran-based micelles for methotrexate delivery |
| title_fullStr | Dually sensitive dextran-based micelles for methotrexate delivery |
| title_full_unstemmed | Dually sensitive dextran-based micelles for methotrexate delivery |
| title_short | Dually sensitive dextran-based micelles for methotrexate delivery |
| title_sort | dually sensitive dextran-based micelles for methotrexate delivery |
| url | https://eprints.nottingham.ac.uk/44129/ https://eprints.nottingham.ac.uk/44129/ https://eprints.nottingham.ac.uk/44129/ |