High-throughput spheroid screens using volume, resazurin reduction and acid phosphatase activity

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High-throughput spheroid screens using volume, resazurin reduction and acid phosphatase activity

Mainstream adoption of physiologically-relevant three-dimensional models has been slow in the last 50 years due to long, manual protocols with poor reproducibility, high price and closed commercial platforms. This chapter describes high-throughput, low-cost, open methods for spheroid viability asses...

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Bibliographic Details
Main Authors:	Ivanov, Delyan Pavlov, Grabowska, Anna M., Garnett, Martin C.
Other Authors:	Gilbert, Daniel F.
Format:	Book Section
Published:	Humana Press (Springer) 2017
Subjects:	alamar blue viability assays overlay culture hanging drop FiJi ImageJ image analysis three-dimensional cell culture in vitro model preclinical screening drug sensitivity
Online Access:	https://eprints.nottingham.ac.uk/43565/

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