Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs

QR Code

Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs

Inducible loss of gene function experiments are necessary to uncover mechanisms underlying development, physiology and disease. However, current methods are complex, lack robustness and do not work in multiple cell types. Here we address these limitations by developing single-step optimized inducibl...

Full description

Bibliographic Details
Main Authors:	Bertero, Alessandro, Pawlowski, Matthias, Ortmann, Daniel, Snijders, Kirsten, Yiangou, Loukia, Cardoso de Brito, Miguel, Brown, Stephanie, Bernard, William G., Cooper, James D., Giacomelli, Elisa, Gambardella, Laure, Hannan, Nicholas R.F., Iyer, Dharini, Sampaziotis, Fotios, Serrano, Felipe, Zonneveld, Mariëlle C.F., Sinha, Sanjay, Kotter, Mark, Vallier, Ludovic
Format:	Article
Published:	Company of Biologists 2016
Subjects:	Human pluripotent stem cells shRNA CRISPR/Cas9 OCT4 POU5F1 T brachyury DPY30
Online Access:	https://eprints.nottingham.ac.uk/43035/

Similar Items

Measurement, reconstruction and flow-field computation of the human pharynx with application to sleep apnea
by: Lucey, Anthony, et al.
Published: (2010)

Targeting HOXA in engineered iPSCs and pre-clinical leukaemia models
by: Abuhantash, Mays
Published: (2023)

Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells
by: Sampaziotis, Fotios, et al.
Published: (2017)

A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery
by: Gu, Y., et al.
Published: (2017)

Effect of shRNA mediated silencing of YB-1 protein on the expression of matrix collagenases in malignant melanoma cell in vitro
by: Ibrahim, Wisam Nabeel, et al.
Published: (2018)

Molecular level investigations of coiled-coil proteins
by: Johnson, Daniel
Published: (2006)

Development of a human induced pluripotent stem cells (hiPSCs)-derived, universal platform, for generation of immunocompetent multi-tissue organoids
by: Azis, Rizal
Published: (2024)

Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids
by: Sampaziotis, Fotios, et al.
Published: (2017)

Injectable supramolecular hydrogel formed from a-cyclodextrin and PEGylated arginine-functionalized poly(L-lysine) dendron for sustained MMP-9 shRNA plasmid delivery
by: Lin, Qun, et al.
Published: (2017)

In vitro modelling of respiratory infections in idiopathic pulmonary fibrosis (IPF) using human induced pluripotent stem cell (hiPSCs)-derived alveolar epithelial type II cells
by: Serna Valverde, Ana Lilia
Published: (2023)

Attempts to create α-synuclein phosphorylation mutant constructs using site-directed mutagenesis and to create γ-synuclein gene silencing constructs using shRNA cloning method.
by: Lee, Yi Heng
Published: (2013)

Organisational Lessons from Jazz Musicians: It Don't Mean A Thing (If It Ain't Got That Swing).
by: Legair, Marielle
Published: (2007)

Revealing the potential role of hsa-miR-663a in modulating the PI3K-Akt signaling pathway via miRNA microarray in spinal muscular atrophy patient fibroblast-derived iPSCs
by: Gandhi, Gayatri, et al.
Published: (2024)

Palaeoecology of Jurassic encrinites: reconstructing crinoid communities from the Western Interior Seaway of North America
by: Hunter, Aaron, et al.
Published: (2008)

Bayesian tolerance intervals with probability matching priors / Dharini a/p Pathmanathan
by: Pathmanathan, Dharini
Published: (2014)

Problems encountered with telecommunication sales and service to refugees and humanitarian migrants in Perth’s northern suburbs – the tip of the iceberg?
by: Snijders, A., et al.
Published: (2014)

On the interpretation of giving, taking and destruction in dictator games and joy-of-destruction games
by: Zhang, Lyla, et al.
Published: (2013)

Exploring the meaning of significance in experimental economics
by: Zhang, Lyla, et al.
Published: (2013)

A reproduction and replication of Engel's meta-study of dictator game experiments
by: Zhang, Lyla, et al.
Published: (2012)

Pro-social or anti-social, or both? A within- and between-subjects study of social preferences
by: Zhang, Lyla, et al.
Published: (2016)

Passenger Classification for an Airport Movement Forecast System
by: Richter, S., et al.
Published: (2009)

Financial market predictions with deep learning
by: Yap, Zhong Jing, et al.
Published: (2023)

Enhancing herbage growth, yield and quality of stevia (stevia rebaudiana bertoni) using bio-organic nutrients in varied soil media
by: Calapardo, Mara Jean Marielle, et al.
Published: (2024)

Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation
by: Sampaziotis, Fotios, et al.
Published: (2015)

Extending the GLM framework of the Lee-Carter model with random forest recursive feature elimination based determinants of mortality
by: Nurul Aityqah Yaacob,, et al.
Published: (2022)

Simplified input-output inversion control of a double pendulum overhead crane for residual oscillations reduction
by: Giacomelli, M., et al.
Published: (2018)

Handling Constraints in Combinatorial Interaction Testing in the Presence of Multi Objective Particle Swarm and Multithreading
by: Ahmed, Bestoun S., et al.
Published: (2017)

Enhancing stock price data analysis through variants of principal component analysis
by: Seow, Teck Xiang, et al.
Published: (2024)

RFE-based feature selection to improve classification accuracy for morphometric analysis of craniodental characters of house rats
by: Aneesha Balachandran Pillay,, et al.
Published: (2023)

Optimum Design of PIλDμ Controller for an Automatic Voltage Regulator System Using Combinatorial Test Design
by: Ahmed, Bestoun S., et al.
Published: (2016)

A new discordancy test on a regression for cylindrical data
by: Nurul Hidayah Sadikon,, et al.
Published: (2018)

Morphometric analysis of craniodental characters of the House Rat, Rattus rattus (Rodentia: Muridae) in Peninsular Malaysia
by: Nurul Huda Mohamad Ikbal,, et al.
Published: (2019)

A model-based control scheme for depth of hypnosis in anesthesia
by: Merigo, L., et al.
Published: (2018)

Phylogenetic relationships and morphological variation of gymnures (Galericidae: Hylomys) from Genting Highlands, Pahang, Malaysia
by: Muhammad Farhan Abd Wahab,, et al.
Published: (2022)

Intramyocellular lipid content and lipogenic gene expression responses following a single bout of resistance type exercise differ between young and older men
by: Tsintzas, Kostas, et al.
Published: (2017)

Investigating AI imitative capabilities: a comparative genre analysis of Reddit and ChatGPT-4 spouse appreciation posts
by: Arcenal, Erika Kristine Estanislao, et al.
Published: (2025)

Messinian age and savannah environment of the possible hominin Graecopithecus from Europe
by: Böhme, M., et al.
Published: (2017)

Antiferromagnetic CuMnAs multi-level memory cell with microelectronic compatibility
by: Olejnik, K., et al.
Published: (2017)

MUSCLEMOTION: a versatile open software tool to quantify cardiomyocyte and cardiac muscle contraction in vitro and in vivo
by: Sala, L., et al.
Published: (2017)

Minimum Information about a Biosynthetic Gene cluster
by: Medema, M., et al.
Published: (2015)