Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs

Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs

Inducible loss of gene function experiments are necessary to uncover mechanisms underlying development, physiology and disease. However, current methods are complex, lack robustness and do not work in multiple cell types. Here we address these limitations by developing single-step optimized inducibl...

Full description

Bibliographic Details
Main Authors: Bertero, Alessandro, Pawlowski, Matthias, Ortmann, Daniel, Snijders, Kirsten, Yiangou, Loukia, Cardoso de Brito, Miguel, Brown, Stephanie, Bernard, William G., Cooper, James D., Giacomelli, Elisa, Gambardella, Laure, Hannan, Nicholas R.F., Iyer, Dharini, Sampaziotis, Fotios, Serrano, Felipe, Zonneveld, Mariëlle C.F., Sinha, Sanjay, Kotter, Mark, Vallier, Ludovic
Format: Article
Published: Company of Biologists 2016
Subjects:
Human pluripotent stem cells
shRNA
CRISPR/Cas9
OCT4
POU5F1
T
brachyury
DPY30
Online Access:https://eprints.nottingham.ac.uk/43035/

Internet

https://eprints.nottingham.ac.uk/43035/

Similar Items