Screening for novel endogenous inflammatory stimuli using the secreted embryonic alkaline phosphatase NF-κB reporter assay

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Screening for novel endogenous inflammatory stimuli using the secreted embryonic alkaline phosphatase NF-κB reporter assay

An immune response can be activated by pathogenic stimuli, as well as endogenous danger signals, triggering the activation of pattern recognition receptors and initiating signalling cascades that lead to inflammation. This method uses THP1-Blue™ cells, a human monocytic cell line which contains an e...

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Bibliographic Details
Main Authors:	Zuliani-Alvarez, Lorena, Piccinini, Anna M., Midwood, Kim S.
Format:	Article
Published:	Bio-protocol LLC 2017
Subjects:	Innate immunity; TLR4; Tenascin-C; LPS; THP1-Blue™ cells; NF-κB reporter
Online Access:	https://eprints.nottingham.ac.uk/42882/

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