Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides
Molar mass or ‘molecular weight’ is one of the most fundamental parameters describing a macromolecule. Because of their polydisperse nature, polysaccharides are usually described by distributions of molar mass. SEC-MALS (size exclusion chromatography coupled to multi-angle light scattering) is often...
| Main Authors: | , , , , |
|---|---|
| Other Authors: | |
| Format: | Book Section |
| Published: |
Springer
2016
|
| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/42817/ |
| _version_ | 1848796575369789440 |
|---|---|
| author | Harding, Stephen E. Adams, Gary G. Gillis, Richard B. Almutairi, Fahad M. Morris, Gordon A. |
| author2 | Uchiyama, Susumu |
| author_facet | Uchiyama, Susumu Harding, Stephen E. Adams, Gary G. Gillis, Richard B. Almutairi, Fahad M. Morris, Gordon A. |
| author_sort | Harding, Stephen E. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Molar mass or ‘molecular weight’ is one of the most fundamental parameters describing a macromolecule. Because of their polydisperse nature, polysaccharides are usually described by distributions of molar mass. SEC-MALS (size exclusion chromatography coupled to multi-angle light scattering) is often a convenient method of choice, but there are many instances where it is unsuitable. Modern AUC (analytical ultracentrifuge) methods provide a valuable alternative – now easier to use than before – and, after briefly reviewing some older procedures, we highlight two recently published and complementary methods, namely, the ‘Extended Fujita’ approach for the analysis of sedimentation velocity data and SEDFIT-MSTAR for the analysis of sedimentation equilibrium data. Nonideality needs to be considered and can be dealt with in a standard way. These methods can also indicate if associative phenomena are present, which can then be quantified using more complex AUC algorithms. |
| first_indexed | 2025-11-14T19:50:10Z |
| format | Book Section |
| id | nottingham-42817 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:50:10Z |
| publishDate | 2016 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-428172020-05-04T17:53:14Z https://eprints.nottingham.ac.uk/42817/ Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides Harding, Stephen E. Adams, Gary G. Gillis, Richard B. Almutairi, Fahad M. Morris, Gordon A. Molar mass or ‘molecular weight’ is one of the most fundamental parameters describing a macromolecule. Because of their polydisperse nature, polysaccharides are usually described by distributions of molar mass. SEC-MALS (size exclusion chromatography coupled to multi-angle light scattering) is often a convenient method of choice, but there are many instances where it is unsuitable. Modern AUC (analytical ultracentrifuge) methods provide a valuable alternative – now easier to use than before – and, after briefly reviewing some older procedures, we highlight two recently published and complementary methods, namely, the ‘Extended Fujita’ approach for the analysis of sedimentation velocity data and SEDFIT-MSTAR for the analysis of sedimentation equilibrium data. Nonideality needs to be considered and can be dealt with in a standard way. These methods can also indicate if associative phenomena are present, which can then be quantified using more complex AUC algorithms. Springer Uchiyama, Susumu Arisaka, Fumio Stafford, Walter F. Laue, Thomas M. 2016-05-04 Book Section PeerReviewed Harding, Stephen E., Adams, Gary G., Gillis, Richard B., Almutairi, Fahad M. and Morris, Gordon A. (2016) Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides. In: Analytical ultracentrifugation: instrumentation, software and applications. Springer, Japan, pp. 375-386. ISBN 978-4-431-55983-2 Polydispersity Nonideality Sedimentation velocity Sedimentation equilibrium http://link.springer.com/chapter/10.1007%2F978-4-431-55985-6_18 doi:10.1007/978-4-431-55985-6_18 doi:10.1007/978-4-431-55985-6_18 |
| spellingShingle | Polydispersity Nonideality Sedimentation velocity Sedimentation equilibrium Harding, Stephen E. Adams, Gary G. Gillis, Richard B. Almutairi, Fahad M. Morris, Gordon A. Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides |
| title | Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides |
| title_full | Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides |
| title_fullStr | Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides |
| title_full_unstemmed | Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides |
| title_short | Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides |
| title_sort | aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides |
| topic | Polydispersity Nonideality Sedimentation velocity Sedimentation equilibrium |
| url | https://eprints.nottingham.ac.uk/42817/ https://eprints.nottingham.ac.uk/42817/ https://eprints.nottingham.ac.uk/42817/ |