A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology

This short review considers the range of modern techniques for the hydrodynamic characterisation of macromolecules – particularly large glycosylated systems used in the food, biopharma and healthcare industries. The range or polydispersity of molecular weights and conformations presents special chal...

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Main Authors: Gillis, Richard B., Rowe, Arthur J., Adams, Gary G., Harding, Stephen E.
Format: Article
Published: Taylor & Francis 2015
Subjects:
Online Access:https://eprints.nottingham.ac.uk/42765/
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author Gillis, Richard B.
Rowe, Arthur J.
Adams, Gary G.
Harding, Stephen E.
author_facet Gillis, Richard B.
Rowe, Arthur J.
Adams, Gary G.
Harding, Stephen E.
author_sort Gillis, Richard B.
building Nottingham Research Data Repository
collection Online Access
description This short review considers the range of modern techniques for the hydrodynamic characterisation of macromolecules – particularly large glycosylated systems used in the food, biopharma and healthcare industries. The range or polydispersity of molecular weights and conformations presents special challenges compared to proteins. The review is aimed, without going into any great theoretical or methodological depth, to help the Industrial Biotechnologist choose the appropriate methodology or combination of methodologies for providing the detail he/she needs for particular applications.
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publishDate 2015
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spelling nottingham-427652020-05-04T17:05:00Z https://eprints.nottingham.ac.uk/42765/ A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology Gillis, Richard B. Rowe, Arthur J. Adams, Gary G. Harding, Stephen E. This short review considers the range of modern techniques for the hydrodynamic characterisation of macromolecules – particularly large glycosylated systems used in the food, biopharma and healthcare industries. The range or polydispersity of molecular weights and conformations presents special challenges compared to proteins. The review is aimed, without going into any great theoretical or methodological depth, to help the Industrial Biotechnologist choose the appropriate methodology or combination of methodologies for providing the detail he/she needs for particular applications. Taylor & Francis 2015-03-05 Article PeerReviewed Gillis, Richard B., Rowe, Arthur J., Adams, Gary G. and Harding, Stephen E. (2015) A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology. Biotechnology and Genetic Engineering Reviews, 30 (2). pp. 142-157. ISSN 0264-8725 analytical ultracentrifugation SEC-MALS viscometry light scattering hydrodynamics biopolymer polydisperse http://www.tandfonline.com/doi/full/10.1080/02648725.2014.994870 doi:10.1080/02648725.2014.994870 doi:10.1080/02648725.2014.994870
spellingShingle analytical ultracentrifugation
SEC-MALS
viscometry
light scattering
hydrodynamics
biopolymer
polydisperse
Gillis, Richard B.
Rowe, Arthur J.
Adams, Gary G.
Harding, Stephen E.
A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology
title A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology
title_full A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology
title_fullStr A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology
title_full_unstemmed A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology
title_short A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology
title_sort review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology
topic analytical ultracentrifugation
SEC-MALS
viscometry
light scattering
hydrodynamics
biopolymer
polydisperse
url https://eprints.nottingham.ac.uk/42765/
https://eprints.nottingham.ac.uk/42765/
https://eprints.nottingham.ac.uk/42765/