Real-time analysis of the binding of fluorescent VEGF₁₆₅a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexes

QR Code

Real-time analysis of the binding of fluorescent VEGF₁₆₅a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexes

Vascular endothelial growth factor (VEGF) is an important mediator of angiogenesis. Here we have used a novel stoichiometric protein-labeling method to generate a fluorescent variant of VEGF (VEGF₁₆₅a-TMR) labeled on a single cysteine within each protomer of the antiparallel VEGF homodimer. VEGF₁₆₅a...

Full description

Bibliographic Details
Main Authors:	Kilpatrick, Laura E., Friedman-Ohana, Rachel, Alcobia, Diana C., Riching, Kristin, Peach, Chloe J., Wheal, Amanda J., Briddon, Stephen J., Robers, Matthew B., Zimmerman, Kris, Machleidt, Thomas, Wood, Keith V., Woolard, Jeanette, Hill, Stephen J.
Format:	Article
Published:	Elsevier 2017
Subjects:	VEGF; VEGFR2; BRET; Ligand binding; Receptor tyrosine kinase inhibitors
Online Access:	https://eprints.nottingham.ac.uk/42120/

Similar Items

Real-time ligand binding of fluorescent VEGF-A isoforms that discriminate between VEGFR2 and NRP1 in living cells
by: Peach, Chloe J., et al.
Published: (2018)

Molecular pharmacology of VEGF-A isoforms: binding and signalling at VEGFR2
by: Peach, Chloe, et al.
Published: (2018)

NanoBRET to probe ligand-receptor and receptor-receptor interactions in living cells
by: Carvalheira Alcobia, Diana
Published: (2019)

Effects of 4 multitargeted receptor tyrosine kinase inhibitors on regional hemodynamics in conscious, freely moving rats
by: Carter, Joanne J., et al.
Published: (2016)

Effects of receptor tyrosine kinase inhibitors on VEGF165a- and VEGF165b-stimulated gene transcription in HEK-293 cells expressing human VEGFR2
by: Carter, Joanne J., et al.
Published: (2015)

Cardiovascular safety liabilities of VEGFR-2 inhibitors
by: Pannucci, Patrizia
Published: (2024)

Synthesis and evaluation of the first fluorescent antagonists of the human P2Y2 receptor based on AR-C118925
by: Conroy, Sean, et al.
Published: (2018)

Allosteric interactions at adenosine A1 and A3 receptors: new insights into the role of small molecules and receptor dimerization
by: Hill, Stephen J., et al.
Published: (2014)

VEGFR2 promotes central endothelial activation and the spread of pain in inflammatory arthritis
by: Beazley-Long, Nicholas, et al.
Published: (2018)

Kinetic analysis of antagonist-occupied adenosine-A3 receptors within membrane microdomains of individual cells provides evidence of receptor dimerization and allosterism
by: Corriden, Ross, et al.
Published: (2014)

Investigating cooperativity across adenosine A3 receptor homo- and hetero-oligomers
by: Groenewoud, N.J.A.
Published: (2019)

Dynamics of VEGF-A binding at VEGFR2 and NRP1
by: Peach, Chloe J
Published: (2020)

Dynamics of ligand and nanobody binding at CXCR4/EGFR complexes
by: Comez, Dehan
Published: (2025)

Differential regulation of blood flow induced neovascularisation and mural cell recruitment by VEGF and angiopoietin signaling
by: Stone, Oliver A., et al.
Published: (2017)

Effect of a toggle switch mutation in TM6 of the human adenosine A3 receptor on Gi protein-dependent signalling and Gi-independent receptor internalization
by: Stoddart, Leigh A., et al.
Published: (2014)

Fluorescence correlation spectroscopy, combined with bimolecular fluorescence complementation, reveals the effects of β-arrestin complexes and endocytic targeting on the membrane mobility of neuropeptide Y receptors
by: Kilpatrick, Laura E., et al.
Published: (2012)

Dietary supplementation with ferric tyrosine improves zootechnical performance and reduces caecal Campylobacter spp. load in poultry
by: Currie, D., et al.
Published: (2018)

Androgen dependent mechanisms of pro-angiogenic networks in placental and tumor development
by: Metzler, Veronika M., et al.
Published: (2017)

Regulation of human feto-placental endothelial barrier integrity by vascular endothelial growth factors: competitive interplay between VEGF-A165a, VEGF-A165b, PIGF and VE-cadherin
by: Pang, Vincent, et al.
Published: (2017)

The use of fluorescence correlation spectroscopy to characterize the molecular mobility of fluorescently labelled G protein-coupled receptors
by: Kilpatrick, Laura E., et al.
Published: (2016)

Mechanisms regulating angiogenesis underlie seasonal control of pituitary function
by: Castle-Miller, Jennifer, et al.
Published: (2017)

Development of existing and novel tyrosinase inhibitors for skin pigmentation: intradermal delivery, biological evaluation and mechanistic studies
by: Li, Zhiwei
Published: (2023)

Direct visualisation of internalization of the adenosine A3 receptor and localization with arrestin3 using a fluorescent agonist
by: Stoddart, Leigh A., et al.
Published: (2015)

Adenosine-A3 receptors in neutrophil microdomains promote the formation of bacteria-tethering cytonemes
by: Corriden, Ross, et al.
Published: (2013)

Expression of vascular endothelial growth factor (VEGF) and its receptor (VEGFR) in thyroid nodular hyperplasia and papillary thyroid carcinoma (PTC)
by: Razy, Nur Hidayati Mohamad Pakarul
Published: (2017)

Role of VEGF-A in chronic pain
by: Hulse, Richard P.
Published: (2017)

Development Of DNA nanosensors as aptasensor for lysozyme and DNAzyme sensor for zinc ions
by: Wai, Jing Luen
Published: (2020)

Synthesis, biological evaluation, and utility of fluorescent ligands targeting the μ-opioid receptor
by: Schembri, Luke S., et al.
Published: (2015)

Electrochemical determination of L-Tryptophan, L-Tyrosine and L-Cysteine using electrospun carbon nanofibers modified electrode
by: Tang, X., et al.
Published: (2010)

A non-imaging high throughput approach to chemical library screening at the unmodified adenosine-A3 receptor in living cells
by: Arruda, Maria Augusta, et al.
Published: (2017)

A phase 2 trial of decreasing tyrosine kinase inhibitor dose in chronic myeloid leukaemia patients with stable major molecular response: data from the British DESTINY study
by: Clark, Richard E., et al.
Published: (2017)

A G protein-coupled receptor dimer imaging assay reveals selectively modified pharmacology of neuropeptide Y Y1/Y5 receptor heterodimers
by: Kilpatrick, Laura E., et al.
Published: (2015)

Breast cancer induced nociceptor aberrant growth and collateral sensory axonal branching
by: Austin, Matt, et al.
Published: (2017)

Effects of VEGF-A165b and SRPK1 inhibition on pain behaviour, cyclooxygenase expression and glial activation in the CNS in a model of osteoarthritis
by: Almahasneh, Fatimah
Published: (2018)

Monoclonal anti-β1-adrenergic receptor antibodies activate G protein signaling in the absence of β-arrestin recruitment
by: Hutchings, Catherine J., et al.
Published: (2013)

Conversion of a non-selective adenosine receptor antagonist into A3-selective high affinity fluorescent probes using peptide-based linkers
by: Vernall, Andrea J., et al.
Published: (2013)

Development of novel fluorescent histamine H₁-receptor antagonists to study ligand-binding kinetics in living cells
by: Stoddart, Leigh A., et al.
Published: (2018)

The effects of EPHA2 inhibition on VEGF, VEGFR-1 and VEGFR-2 in human malignant glioma cells
by: Baharuddin, Wan Noor Ainun
Published: (2018)

Can bioluminescence resonance energy transfer be used to elucidate structural and binding information about the ABCG2 transporter?
by: Morris, Joseph
Published: (2022)

Peripheral contributions to the development and maintenance of inflammation and pain in arthritis
by: Amanitis, Dimitrios
Published: (2022)