Real-time analysis of the binding of fluorescent VEGF₁₆₅a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexes

Real-time analysis of the binding of fluorescent VEGF₁₆₅a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexes

Vascular endothelial growth factor (VEGF) is an important mediator of angiogenesis. Here we have used a novel stoichiometric protein-labeling method to generate a fluorescent variant of VEGF (VEGF₁₆₅a-TMR) labeled on a single cysteine within each protomer of the antiparallel VEGF homodimer. VEGF₁₆₅a...

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Bibliographic Details
Main Authors: Kilpatrick, Laura E., Friedman-Ohana, Rachel, Alcobia, Diana C., Riching, Kristin, Peach, Chloe J., Wheal, Amanda J., Briddon, Stephen J., Robers, Matthew B., Zimmerman, Kris, Machleidt, Thomas, Wood, Keith V., Woolard, Jeanette, Hill, Stephen J.
Format: Article
Published: Elsevier 2017
Subjects:
VEGF; VEGFR2; BRET; Ligand binding; Receptor tyrosine kinase inhibitors
Online Access:https://eprints.nottingham.ac.uk/42120/

Internet

https://eprints.nottingham.ac.uk/42120/

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