Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus

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Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus

Single-stranded DNA-binding protein (SSB) is a well characterized ubiquitous and essential bacterial protein involved in almost all aspects of DNA metabolism. Using the Bacillus subtilis SSB we have generated a reagentless SSB biosensor that can be used as a helicase probe in B. subtilis and closely...

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Bibliographic Details
Main Authors:	Green, Matthew, Gilhooly, Neville S., Abedeen, Shahriar, Scott, David J., Dillingham, Mark S., Soultanas, Panos
Format:	Article
Language:	English
Published:	Elsevier 2014
Subjects:	SSB; Helicases; Fluorescence; DNA unwinding; Bacillus subtilis; Firmicutes
Online Access:	https://eprints.nottingham.ac.uk/41969/

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