Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods
Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bacterial polysaccharides or glycoconjugates in solution. This chapter focuses on the following hydrodynamic methods: sedimentation velocity analytical ultracentrifugation (SV AUC), dynamic light scattering...
| Main Authors: | , , , , |
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| Format: | Book Section |
| Published: |
Springer
2015
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| Online Access: | https://eprints.nottingham.ac.uk/41724/ |
| _version_ | 1848796340271710208 |
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| author | Harding, Stephen E. Abdelhameed, Ali Saber Gillis, Richard B. Morris, Gordon A. Adams, Gary G. |
| author_facet | Harding, Stephen E. Abdelhameed, Ali Saber Gillis, Richard B. Morris, Gordon A. Adams, Gary G. |
| author_sort | Harding, Stephen E. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bacterial polysaccharides or glycoconjugates in solution. This chapter focuses on the following hydrodynamic methods: sedimentation velocity analytical ultracentrifugation (SV AUC), dynamic light scattering (DLS), sedimentation equilibrium analytical ultracentrifugation (SE AUC), size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), and capillary viscometry—intrinsic viscosity measurement. The chapter highlights the general principle of these five methods, describes experimental details, and specifies advances in the last years. |
| first_indexed | 2025-11-14T19:46:25Z |
| format | Book Section |
| id | nottingham-41724 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:46:25Z |
| publishDate | 2015 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-417242020-05-04T20:11:00Z https://eprints.nottingham.ac.uk/41724/ Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods Harding, Stephen E. Abdelhameed, Ali Saber Gillis, Richard B. Morris, Gordon A. Adams, Gary G. Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bacterial polysaccharides or glycoconjugates in solution. This chapter focuses on the following hydrodynamic methods: sedimentation velocity analytical ultracentrifugation (SV AUC), dynamic light scattering (DLS), sedimentation equilibrium analytical ultracentrifugation (SE AUC), size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), and capillary viscometry—intrinsic viscosity measurement. The chapter highlights the general principle of these five methods, describes experimental details, and specifies advances in the last years. Springer 2015 Book Section PeerReviewed Harding, Stephen E., Abdelhameed, Ali Saber, Gillis, Richard B., Morris, Gordon A. and Adams, Gary G. (2015) Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods. In: Carbohydrate-based vaccines. Methods in molecular biology (1331). Springer, pp. 211-227. ISBN 9781493928743 https://link.springer.com/protocol/10.1007%2F978-1-4939-2874-3_13 doi:10.1007/978-1-4939-2874-3_13 doi:10.1007/978-1-4939-2874-3_13 |
| spellingShingle | Harding, Stephen E. Abdelhameed, Ali Saber Gillis, Richard B. Morris, Gordon A. Adams, Gary G. Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods |
| title | Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods |
| title_full | Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods |
| title_fullStr | Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods |
| title_full_unstemmed | Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods |
| title_short | Characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods |
| title_sort | characterization of capsular polysaccharides and their glycoconjugates by hydrodynamic methods |
| url | https://eprints.nottingham.ac.uk/41724/ https://eprints.nottingham.ac.uk/41724/ https://eprints.nottingham.ac.uk/41724/ |