Matrix-free hydrodynamic study on the size distribution and conformation of three technical lignins from wood and non-wood

Molecular weight (MW) and related conformational data of three commercially available technical lignins (Alcell L, kraft L, and soda L) have been studied by means of analytical ultracentrifugation, taking advantage of some recent developments in both sedimentation velocity and sedimentation equilibr...

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Main Authors: Alzahrani, Qushmua E., Adams, Gary G., Gillis, Richard B., Besong, Tabot M.D., Kök, M. Samil, Fong, Emily, Harding, Richard A., van Dam, Jan E.G., Gosselink, Richard J.A., Rowe, Arthur J., Harding, Stephen E.
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Published: De Gruyter 2016
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Online Access:https://eprints.nottingham.ac.uk/41702/
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author Alzahrani, Qushmua E.
Adams, Gary G.
Gillis, Richard B.
Besong, Tabot M.D.
Kök, M. Samil
Fong, Emily
Harding, Richard A.
van Dam, Jan E.G.
Gosselink, Richard J.A.
Rowe, Arthur J.
Harding, Stephen E.
author_facet Alzahrani, Qushmua E.
Adams, Gary G.
Gillis, Richard B.
Besong, Tabot M.D.
Kök, M. Samil
Fong, Emily
Harding, Richard A.
van Dam, Jan E.G.
Gosselink, Richard J.A.
Rowe, Arthur J.
Harding, Stephen E.
author_sort Alzahrani, Qushmua E.
building Nottingham Research Data Repository
collection Online Access
description Molecular weight (MW) and related conformational data of three commercially available technical lignins (Alcell L, kraft L, and soda L) have been studied by means of analytical ultracentrifugation, taking advantage of some recent developments in both sedimentation velocity and sedimentation equilibrium determinations. The lignins were dissolved in dimethyl sulphoxide (with ca. 90% solubility), and solutions were studied with regards to their oligomeric state, heterogeneity profiles (distribution of sedimentation coefficients), and molecular weight distributions (MWD). Alcell L and soda L have similar properties showing one major low MW component and two minor high MW components, whereas kraft L appears to be larger and more uniform, i.e., it shows a more monodisperse MWD. Weight average molecular weight (Mw) data from sedimentation equilibrium obtained by the new SEDFIT-MSTAR procedure in conjunction with MULTISIG analysis were found to be ~18 kDa (Alcell L), 25 kDa (kraft L), and 15 kDa (soda L). Further analysis of the data by means of the routines MULTISIG and M_INVEQ confirmed the presence of additional components in Alcell L and soda L, and the larger size and high degree of monodispersity of kraft L. The intrinsic viscosity data of the three lignins were found to be very similar in the range of 22–24 ml g-1, and all data were consistent with an elongated plate shape molecular structure with an equivalent discoid aspect ratio ~30.
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spelling nottingham-417022020-05-04T17:28:29Z https://eprints.nottingham.ac.uk/41702/ Matrix-free hydrodynamic study on the size distribution and conformation of three technical lignins from wood and non-wood Alzahrani, Qushmua E. Adams, Gary G. Gillis, Richard B. Besong, Tabot M.D. Kök, M. Samil Fong, Emily Harding, Richard A. van Dam, Jan E.G. Gosselink, Richard J.A. Rowe, Arthur J. Harding, Stephen E. Molecular weight (MW) and related conformational data of three commercially available technical lignins (Alcell L, kraft L, and soda L) have been studied by means of analytical ultracentrifugation, taking advantage of some recent developments in both sedimentation velocity and sedimentation equilibrium determinations. The lignins were dissolved in dimethyl sulphoxide (with ca. 90% solubility), and solutions were studied with regards to their oligomeric state, heterogeneity profiles (distribution of sedimentation coefficients), and molecular weight distributions (MWD). Alcell L and soda L have similar properties showing one major low MW component and two minor high MW components, whereas kraft L appears to be larger and more uniform, i.e., it shows a more monodisperse MWD. Weight average molecular weight (Mw) data from sedimentation equilibrium obtained by the new SEDFIT-MSTAR procedure in conjunction with MULTISIG analysis were found to be ~18 kDa (Alcell L), 25 kDa (kraft L), and 15 kDa (soda L). Further analysis of the data by means of the routines MULTISIG and M_INVEQ confirmed the presence of additional components in Alcell L and soda L, and the larger size and high degree of monodispersity of kraft L. The intrinsic viscosity data of the three lignins were found to be very similar in the range of 22–24 ml g-1, and all data were consistent with an elongated plate shape molecular structure with an equivalent discoid aspect ratio ~30. De Gruyter 2016-02-01 Article PeerReviewed Alzahrani, Qushmua E., Adams, Gary G., Gillis, Richard B., Besong, Tabot M.D., Kök, M. Samil, Fong, Emily, Harding, Richard A., van Dam, Jan E.G., Gosselink, Richard J.A., Rowe, Arthur J. and Harding, Stephen E. (2016) Matrix-free hydrodynamic study on the size distribution and conformation of three technical lignins from wood and non-wood. Holzforschung, 70 (2). ISSN 0018-3830 Alcell L; analytical ultracentrifugation; disc shape; DMSO; kraft L; soda L; viscometry https://www.degruyter.com/view/j/hfsg.2016.70.issue-2/hf-2014-0318/hf-2014-0318.xml doi:10.1515/hf-2014-0318 doi:10.1515/hf-2014-0318
spellingShingle Alcell L; analytical ultracentrifugation; disc shape; DMSO; kraft L; soda L; viscometry
Alzahrani, Qushmua E.
Adams, Gary G.
Gillis, Richard B.
Besong, Tabot M.D.
Kök, M. Samil
Fong, Emily
Harding, Richard A.
van Dam, Jan E.G.
Gosselink, Richard J.A.
Rowe, Arthur J.
Harding, Stephen E.
Matrix-free hydrodynamic study on the size distribution and conformation of three technical lignins from wood and non-wood
title Matrix-free hydrodynamic study on the size distribution and conformation of three technical lignins from wood and non-wood
title_full Matrix-free hydrodynamic study on the size distribution and conformation of three technical lignins from wood and non-wood
title_fullStr Matrix-free hydrodynamic study on the size distribution and conformation of three technical lignins from wood and non-wood
title_full_unstemmed Matrix-free hydrodynamic study on the size distribution and conformation of three technical lignins from wood and non-wood
title_short Matrix-free hydrodynamic study on the size distribution and conformation of three technical lignins from wood and non-wood
title_sort matrix-free hydrodynamic study on the size distribution and conformation of three technical lignins from wood and non-wood
topic Alcell L; analytical ultracentrifugation; disc shape; DMSO; kraft L; soda L; viscometry
url https://eprints.nottingham.ac.uk/41702/
https://eprints.nottingham.ac.uk/41702/
https://eprints.nottingham.ac.uk/41702/