IL-36 receptor is expressed by human blood and intestinal T lymphocytes and is dose–dependently activated via IL-36β and induces CD4+ lymphocyte proliferation

We show that IL-36R is expressed by T (CD4+ and CD8+) and B (CD19+) lymphocytes in human blood and also by CD4+ T lymphocytes in the intestinal lamina propria. IL-36R protein was mostly stored in the cytoplasm of CD4 lymphocytes and B cells, during steady state conditions and the greatest expression...

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Main Authors: Penha, Rafael, Higgins, John, Mutamba, Shilla, Barrow, Paul, Mahida, Yashwant R., Foster, Neil
Format: Article
Published: Elsevier 2016
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Online Access:https://eprints.nottingham.ac.uk/41582/
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author Penha, Rafael
Higgins, John
Mutamba, Shilla
Barrow, Paul
Mahida, Yashwant R.
Foster, Neil
author_facet Penha, Rafael
Higgins, John
Mutamba, Shilla
Barrow, Paul
Mahida, Yashwant R.
Foster, Neil
author_sort Penha, Rafael
building Nottingham Research Data Repository
collection Online Access
description We show that IL-36R is expressed by T (CD4+ and CD8+) and B (CD19+) lymphocytes in human blood and also by CD4+ T lymphocytes in the intestinal lamina propria. IL-36R protein was mostly stored in the cytoplasm of CD4 lymphocytes and B cells, during steady state conditions and the greatest expression of IL-36R mRNA was measured in CD4+ (T helper) lymphocytes. IL-36 β, which functions via IL-36R induced rapid and significant (P < 0.05) proliferation of CD4+ lymphocytes, within 48 h. IL-36R expression was also maintained on the surface of circulating CD4+ lymphocytes which enter the intestinal lamina propria. In conclusion our study is the first to show that (1) all human blood lymphocytes express IL-36R; (2) IL-36R expression is maintained by circulating CD4+ lymphocytes which enter the intestinal lamina propria and (3) IL-36R/IL-36 β induces rapid CD4 lymphocyte proliferation. The possible significance of these results in the context of human disease is discussed.
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spelling nottingham-415822020-05-04T18:08:56Z https://eprints.nottingham.ac.uk/41582/ IL-36 receptor is expressed by human blood and intestinal T lymphocytes and is dose–dependently activated via IL-36β and induces CD4+ lymphocyte proliferation Penha, Rafael Higgins, John Mutamba, Shilla Barrow, Paul Mahida, Yashwant R. Foster, Neil We show that IL-36R is expressed by T (CD4+ and CD8+) and B (CD19+) lymphocytes in human blood and also by CD4+ T lymphocytes in the intestinal lamina propria. IL-36R protein was mostly stored in the cytoplasm of CD4 lymphocytes and B cells, during steady state conditions and the greatest expression of IL-36R mRNA was measured in CD4+ (T helper) lymphocytes. IL-36 β, which functions via IL-36R induced rapid and significant (P < 0.05) proliferation of CD4+ lymphocytes, within 48 h. IL-36R expression was also maintained on the surface of circulating CD4+ lymphocytes which enter the intestinal lamina propria. In conclusion our study is the first to show that (1) all human blood lymphocytes express IL-36R; (2) IL-36R expression is maintained by circulating CD4+ lymphocytes which enter the intestinal lamina propria and (3) IL-36R/IL-36 β induces rapid CD4 lymphocyte proliferation. The possible significance of these results in the context of human disease is discussed. Elsevier 2016-09-30 Article PeerReviewed Penha, Rafael, Higgins, John, Mutamba, Shilla, Barrow, Paul, Mahida, Yashwant R. and Foster, Neil (2016) IL-36 receptor is expressed by human blood and intestinal T lymphocytes and is dose–dependently activated via IL-36β and induces CD4+ lymphocyte proliferation. Cytokine, 85 . pp. 18-25. ISSN 1096-0023 IL-36; IL-36R; Human; Lymphocyte https://doi.org/10.1016/j.cyto.2016.05.023 doi:10.1016/j.cyto.2016.05.023 doi:10.1016/j.cyto.2016.05.023
spellingShingle IL-36; IL-36R; Human; Lymphocyte
Penha, Rafael
Higgins, John
Mutamba, Shilla
Barrow, Paul
Mahida, Yashwant R.
Foster, Neil
IL-36 receptor is expressed by human blood and intestinal T lymphocytes and is dose–dependently activated via IL-36β and induces CD4+ lymphocyte proliferation
title IL-36 receptor is expressed by human blood and intestinal T lymphocytes and is dose–dependently activated via IL-36β and induces CD4+ lymphocyte proliferation
title_full IL-36 receptor is expressed by human blood and intestinal T lymphocytes and is dose–dependently activated via IL-36β and induces CD4+ lymphocyte proliferation
title_fullStr IL-36 receptor is expressed by human blood and intestinal T lymphocytes and is dose–dependently activated via IL-36β and induces CD4+ lymphocyte proliferation
title_full_unstemmed IL-36 receptor is expressed by human blood and intestinal T lymphocytes and is dose–dependently activated via IL-36β and induces CD4+ lymphocyte proliferation
title_short IL-36 receptor is expressed by human blood and intestinal T lymphocytes and is dose–dependently activated via IL-36β and induces CD4+ lymphocyte proliferation
title_sort il-36 receptor is expressed by human blood and intestinal t lymphocytes and is dose–dependently activated via il-36β and induces cd4+ lymphocyte proliferation
topic IL-36; IL-36R; Human; Lymphocyte
url https://eprints.nottingham.ac.uk/41582/
https://eprints.nottingham.ac.uk/41582/
https://eprints.nottingham.ac.uk/41582/