Viabilité et quantification d’une levure probiotique dans le système digestif du porc

Viability and colonisation of a probiotic yeast in the digestive tract of piglets Probiotics have been defined as « live microorganisms which when administered in adequate amounts confer a health benefit on the host» (WHO, 2001). This definition implies that viability is an important factor and t...

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Main Authors: Le Bon, Melanie, Bravo de Laguna, Fernando, Chevaux, Eric, Mellits, Kenneth H.
Format: Article
Published: IFIP (Institut du porc) 2016
Online Access:https://eprints.nottingham.ac.uk/41022/
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author Le Bon, Melanie
Bravo de Laguna, Fernando
Chevaux, Eric
Mellits, Kenneth H.
author_facet Le Bon, Melanie
Bravo de Laguna, Fernando
Chevaux, Eric
Mellits, Kenneth H.
author_sort Le Bon, Melanie
building Nottingham Research Data Repository
collection Online Access
description Viability and colonisation of a probiotic yeast in the digestive tract of piglets Probiotics have been defined as « live microorganisms which when administered in adequate amounts confer a health benefit on the host» (WHO, 2001). This definition implies that viability is an important factor and that the probiotic needs to reach its target site alive and in significant number in order to confer beneficial properties. Therefore, we investigated the survival and the level of the probiotic yeast Saccharomyces cerevisiae CNCM I-1079 (SB) along the gut of pigs and in faeces. For this purpose, 3 piglets from SB supplemented sows were orally dosed with SB for a week before sacrifice at 21 days of age. Faecal samples were collected as well as intestinal tract compartment content for yeast count and characterization. We have used advanced techniques to be able to identify and enumerate accurately SB from other yeasts according to morphology and biomolecular profile criteria. We have demonstrated that SB remains viable and in proportionally high number along the gut of pigs and in faeces, suggesting that both sites of main actions for probiotics (small intestine and hindgut) can benefit from the presence of live yeast cells at a biologically significant level.
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spelling nottingham-410222020-05-04T17:38:13Z https://eprints.nottingham.ac.uk/41022/ Viabilité et quantification d’une levure probiotique dans le système digestif du porc Le Bon, Melanie Bravo de Laguna, Fernando Chevaux, Eric Mellits, Kenneth H. Viability and colonisation of a probiotic yeast in the digestive tract of piglets Probiotics have been defined as « live microorganisms which when administered in adequate amounts confer a health benefit on the host» (WHO, 2001). This definition implies that viability is an important factor and that the probiotic needs to reach its target site alive and in significant number in order to confer beneficial properties. Therefore, we investigated the survival and the level of the probiotic yeast Saccharomyces cerevisiae CNCM I-1079 (SB) along the gut of pigs and in faeces. For this purpose, 3 piglets from SB supplemented sows were orally dosed with SB for a week before sacrifice at 21 days of age. Faecal samples were collected as well as intestinal tract compartment content for yeast count and characterization. We have used advanced techniques to be able to identify and enumerate accurately SB from other yeasts according to morphology and biomolecular profile criteria. We have demonstrated that SB remains viable and in proportionally high number along the gut of pigs and in faeces, suggesting that both sites of main actions for probiotics (small intestine and hindgut) can benefit from the presence of live yeast cells at a biologically significant level. IFIP (Institut du porc) 2016-02-03 Article PeerReviewed Le Bon, Melanie, Bravo de Laguna, Fernando, Chevaux, Eric and Mellits, Kenneth H. (2016) Viabilité et quantification d’une levure probiotique dans le système digestif du porc. Journées Recherche Porcine, 48 . pp. 133-134. ISSN 0767-9874 http://www.journees-recherche-porcine.com/texte/2016/alimentation/ap18.pdf
spellingShingle Le Bon, Melanie
Bravo de Laguna, Fernando
Chevaux, Eric
Mellits, Kenneth H.
Viabilité et quantification d’une levure probiotique dans le système digestif du porc
title Viabilité et quantification d’une levure probiotique dans le système digestif du porc
title_full Viabilité et quantification d’une levure probiotique dans le système digestif du porc
title_fullStr Viabilité et quantification d’une levure probiotique dans le système digestif du porc
title_full_unstemmed Viabilité et quantification d’une levure probiotique dans le système digestif du porc
title_short Viabilité et quantification d’une levure probiotique dans le système digestif du porc
title_sort viabilité et quantification d’une levure probiotique dans le système digestif du porc
url https://eprints.nottingham.ac.uk/41022/
https://eprints.nottingham.ac.uk/41022/