Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease
5-methylcytosine and the oxidation product 5-hydroxymethylcytosine are two prominent epigenetic variants of the cytosine base in nuclear DNA of mammalian brains. We measured levels of 5-methylcytosine and 5-hydroxymethylcytosine by enzyme-linked immunosorbent assay in DNA from post-mortem cerebella...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Published: |
Nature Publishing Group
2017
|
| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/40271/ |
| _version_ | 1848796022183034880 |
|---|---|
| author | Stöger, Reinhard Scaife, Paula Shephard, Freya Chakrabarti, Lisa |
| author_facet | Stöger, Reinhard Scaife, Paula Shephard, Freya Chakrabarti, Lisa |
| author_sort | Stöger, Reinhard |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | 5-methylcytosine and the oxidation product 5-hydroxymethylcytosine are two prominent epigenetic variants of the cytosine base in nuclear DNA of mammalian brains. We measured levels of 5-methylcytosine and 5-hydroxymethylcytosine by enzyme-linked immunosorbent assay in DNA from post-mortem cerebella of individuals with Parkinson’s disease and age-matched controls. 5-methylcytosine levels showed no significant differences between Parkinson’s disease and control DNA sample sets. In contrast, median 5-hydroxymethylcytosine levels were almost twice as high (p < 0.001) in both male and female Parkinson’s disease individuals compared with controls. The distinct epigenetic profile identified in cerebellar DNA of Parkinson’s disease patients raises the question whether elevated 5-hydroxymethylcytosine levels are a driver or a consequence of Parkinson’s disease. |
| first_indexed | 2025-11-14T19:41:22Z |
| format | Article |
| id | nottingham-40271 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:41:22Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-402712024-08-15T15:21:23Z https://eprints.nottingham.ac.uk/40271/ Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease Stöger, Reinhard Scaife, Paula Shephard, Freya Chakrabarti, Lisa 5-methylcytosine and the oxidation product 5-hydroxymethylcytosine are two prominent epigenetic variants of the cytosine base in nuclear DNA of mammalian brains. We measured levels of 5-methylcytosine and 5-hydroxymethylcytosine by enzyme-linked immunosorbent assay in DNA from post-mortem cerebella of individuals with Parkinson’s disease and age-matched controls. 5-methylcytosine levels showed no significant differences between Parkinson’s disease and control DNA sample sets. In contrast, median 5-hydroxymethylcytosine levels were almost twice as high (p < 0.001) in both male and female Parkinson’s disease individuals compared with controls. The distinct epigenetic profile identified in cerebellar DNA of Parkinson’s disease patients raises the question whether elevated 5-hydroxymethylcytosine levels are a driver or a consequence of Parkinson’s disease. Nature Publishing Group 2017-02-01 Article PeerReviewed Stöger, Reinhard, Scaife, Paula, Shephard, Freya and Chakrabarti, Lisa (2017) Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease. npj Parkinson's Disease, 3 (6). pp. 1-6. ISSN 2373-8057 DNA epigenetics Parkinson's disease http://www.nature.com/articles/s41531-017-0007-3 doi:10.1038/s41531-017-0007-3 doi:10.1038/s41531-017-0007-3 |
| spellingShingle | DNA epigenetics Parkinson's disease Stöger, Reinhard Scaife, Paula Shephard, Freya Chakrabarti, Lisa Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease |
| title | Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease |
| title_full | Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease |
| title_fullStr | Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease |
| title_full_unstemmed | Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease |
| title_short | Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease |
| title_sort | elevated 5hmc levels characterize dna of the cerebellum in parkinson’s disease |
| topic | DNA epigenetics Parkinson's disease |
| url | https://eprints.nottingham.ac.uk/40271/ https://eprints.nottingham.ac.uk/40271/ https://eprints.nottingham.ac.uk/40271/ |