Variation in sulfur and selenium accumulation is controlled by naturally occurring isoforms of the key sulfur assimilation enzyme ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE2 across the arabidopsis species range
Natural variation allows the investigation of both the fundamental functions of genes and their role in local adaptation. As one of the essential macronutrients, sulfur is vital for plant growth and development and also for crop yield and quality. Selenium and sulfur are assimilated by the same proc...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
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American Society of Plant Physiology
2014
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| Online Access: | https://eprints.nottingham.ac.uk/40132/ |
| _version_ | 1848795991150428160 |
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| author | Chao, Dai-Yin Baraniecka, Patrycja Danku, John Koprivova, Anna Lahner, Brett Luo, Hongbong Yakubova, Elena Dilkes, Brian Kopriva, Stanislav Salt, David E. |
| author_facet | Chao, Dai-Yin Baraniecka, Patrycja Danku, John Koprivova, Anna Lahner, Brett Luo, Hongbong Yakubova, Elena Dilkes, Brian Kopriva, Stanislav Salt, David E. |
| author_sort | Chao, Dai-Yin |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Natural variation allows the investigation of both the fundamental functions of genes and their role in local adaptation. As one of the essential macronutrients, sulfur is vital for plant growth and development and also for crop yield and quality. Selenium and sulfur are assimilated by the same process, and although plants do not require selenium, plant-based selenium is an important source of this essential element for animals. Here, we report the use of linkage mapping in synthetic F2 populations and complementation to investigate the genetic architecture of variation in total leaf sulfur and selenium concentrations in a diverse set of Arabidopsis (Arabidopsis thaliana) accessions. We identify in accessions collected from Sweden and the Czech Republic two variants of the enzyme ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE2 (APR2) with strongly diminished catalytic capacity. APR2 is a key enzyme in both sulfate and selenate reduction, and its reduced activity in the loss-of-function allele apr2-1 and the two Arabidopsis accessions Hodonín and Shahdara leads to a lowering of sulfur flux from sulfate into the reduced sulfur compounds, cysteine and glutathione, and into proteins, concomitant with an increase in the accumulation of sulfate in leaves. We conclude from our observation, and the previously identified weak allele of APR2 from the Shahdara accession collected in Tadjikistan, that the catalytic capacity of APR2 varies by 4 orders of magnitude across the Arabidopsis species range, driving significant differences in sulfur and selenium metabolism. The selective benefit, if any, of this large variation remains to be explored. |
| first_indexed | 2025-11-14T19:40:52Z |
| format | Article |
| id | nottingham-40132 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:40:52Z |
| publishDate | 2014 |
| publisher | American Society of Plant Physiology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-401322020-05-04T16:53:38Z https://eprints.nottingham.ac.uk/40132/ Variation in sulfur and selenium accumulation is controlled by naturally occurring isoforms of the key sulfur assimilation enzyme ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE2 across the arabidopsis species range Chao, Dai-Yin Baraniecka, Patrycja Danku, John Koprivova, Anna Lahner, Brett Luo, Hongbong Yakubova, Elena Dilkes, Brian Kopriva, Stanislav Salt, David E. Natural variation allows the investigation of both the fundamental functions of genes and their role in local adaptation. As one of the essential macronutrients, sulfur is vital for plant growth and development and also for crop yield and quality. Selenium and sulfur are assimilated by the same process, and although plants do not require selenium, plant-based selenium is an important source of this essential element for animals. Here, we report the use of linkage mapping in synthetic F2 populations and complementation to investigate the genetic architecture of variation in total leaf sulfur and selenium concentrations in a diverse set of Arabidopsis (Arabidopsis thaliana) accessions. We identify in accessions collected from Sweden and the Czech Republic two variants of the enzyme ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE2 (APR2) with strongly diminished catalytic capacity. APR2 is a key enzyme in both sulfate and selenate reduction, and its reduced activity in the loss-of-function allele apr2-1 and the two Arabidopsis accessions Hodonín and Shahdara leads to a lowering of sulfur flux from sulfate into the reduced sulfur compounds, cysteine and glutathione, and into proteins, concomitant with an increase in the accumulation of sulfate in leaves. We conclude from our observation, and the previously identified weak allele of APR2 from the Shahdara accession collected in Tadjikistan, that the catalytic capacity of APR2 varies by 4 orders of magnitude across the Arabidopsis species range, driving significant differences in sulfur and selenium metabolism. The selective benefit, if any, of this large variation remains to be explored. American Society of Plant Physiology 2014-09-22 Article PeerReviewed Chao, Dai-Yin, Baraniecka, Patrycja, Danku, John, Koprivova, Anna, Lahner, Brett, Luo, Hongbong, Yakubova, Elena, Dilkes, Brian, Kopriva, Stanislav and Salt, David E. (2014) Variation in sulfur and selenium accumulation is controlled by naturally occurring isoforms of the key sulfur assimilation enzyme ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE2 across the arabidopsis species range. Plant Physiology, 166 (3). pp. 1593-1608. ISSN 1532-2548 http://www.plantphysiol.org/content/166/3/1593 doi:10.1104/pp.114.247825 doi:10.1104/pp.114.247825 |
| spellingShingle | Chao, Dai-Yin Baraniecka, Patrycja Danku, John Koprivova, Anna Lahner, Brett Luo, Hongbong Yakubova, Elena Dilkes, Brian Kopriva, Stanislav Salt, David E. Variation in sulfur and selenium accumulation is controlled by naturally occurring isoforms of the key sulfur assimilation enzyme ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE2 across the arabidopsis species range |
| title | Variation in sulfur and selenium accumulation is controlled by naturally occurring isoforms of the key sulfur assimilation enzyme ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE2 across the arabidopsis species range |
| title_full | Variation in sulfur and selenium accumulation is controlled by naturally occurring isoforms of the key sulfur assimilation enzyme ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE2 across the arabidopsis species range |
| title_fullStr | Variation in sulfur and selenium accumulation is controlled by naturally occurring isoforms of the key sulfur assimilation enzyme ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE2 across the arabidopsis species range |
| title_full_unstemmed | Variation in sulfur and selenium accumulation is controlled by naturally occurring isoforms of the key sulfur assimilation enzyme ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE2 across the arabidopsis species range |
| title_short | Variation in sulfur and selenium accumulation is controlled by naturally occurring isoforms of the key sulfur assimilation enzyme ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE2 across the arabidopsis species range |
| title_sort | variation in sulfur and selenium accumulation is controlled by naturally occurring isoforms of the key sulfur assimilation enzyme adenosine 5′-phosphosulfate reductase2 across the arabidopsis species range |
| url | https://eprints.nottingham.ac.uk/40132/ https://eprints.nottingham.ac.uk/40132/ https://eprints.nottingham.ac.uk/40132/ |