Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants
A transaminase from Halomonas elongata and four mutants generated by an in silico-based design, were recombinantly produced in E. coli, purified and applied to the amination of mono-substituted aromatic carbonyl-derivatives. While benzaldehyde derivatives resulted excellent substrates, only NO2-acet...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Published: |
Royal Society of Chemistry
2016
|
| Online Access: | https://eprints.nottingham.ac.uk/38985/ |
| _version_ | 1848795734368845824 |
|---|---|
| author | Contente, Martina Letizia Planchestainer, Matteo Molinari, Francesco Paradisi, Francesca |
| author_facet | Contente, Martina Letizia Planchestainer, Matteo Molinari, Francesco Paradisi, Francesca |
| author_sort | Contente, Martina Letizia |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | A transaminase from Halomonas elongata and four mutants generated by an in silico-based design, were recombinantly produced in E. coli, purified and applied to the amination of mono-substituted aromatic carbonyl-derivatives. While benzaldehyde derivatives resulted excellent substrates, only NO2-acetophenones were transformed into the (S)-amine with high enantioselectivity. The different behaviour of wild-type and mutated transaminases was assessed by in silico substrate binding mode studies. |
| first_indexed | 2025-11-14T19:36:48Z |
| format | Article |
| id | nottingham-38985 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:36:48Z |
| publishDate | 2016 |
| publisher | Royal Society of Chemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-389852020-05-04T18:13:21Z https://eprints.nottingham.ac.uk/38985/ Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants Contente, Martina Letizia Planchestainer, Matteo Molinari, Francesco Paradisi, Francesca A transaminase from Halomonas elongata and four mutants generated by an in silico-based design, were recombinantly produced in E. coli, purified and applied to the amination of mono-substituted aromatic carbonyl-derivatives. While benzaldehyde derivatives resulted excellent substrates, only NO2-acetophenones were transformed into the (S)-amine with high enantioselectivity. The different behaviour of wild-type and mutated transaminases was assessed by in silico substrate binding mode studies. Royal Society of Chemistry 2016-09-06 Article PeerReviewed Contente, Martina Letizia, Planchestainer, Matteo, Molinari, Francesco and Paradisi, Francesca (2016) Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants. Organic and Biomolecular Chemistry, 14 . pp. 9306-9311. ISSN 1477-0539 http://pubs.rsc.org/en/Content/ArticleLanding/2016/OB/C6OB01629D#!divAbstract doi:10.1039/C6OB01629D doi:10.1039/C6OB01629D |
| spellingShingle | Contente, Martina Letizia Planchestainer, Matteo Molinari, Francesco Paradisi, Francesca Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants |
| title | Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants |
| title_full | Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants |
| title_fullStr | Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants |
| title_full_unstemmed | Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants |
| title_short | Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants |
| title_sort | stereoelectronic effects in the reaction of aromatic substrates catalysed by halomonas elongata transaminase and its mutants |
| url | https://eprints.nottingham.ac.uk/38985/ https://eprints.nottingham.ac.uk/38985/ https://eprints.nottingham.ac.uk/38985/ |