Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals
Serological surveillance and vaccination are important strategies for controlling infectious diseases of food production animals. However, the compatibility of these strategies is limited by a lack of assays capable of di erentiating infected from vaccinated animals (DIVA tests) for established kill...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
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Nature Publishing Group
2016
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| Online Access: | https://eprints.nottingham.ac.uk/38514/ |
| _version_ | 1848795629353959424 |
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| author | Naqid, Ibrahim A. Owen, Jonathan P. Maddison, Ben C. Spiliotopoulos, Anastasios Emes, Richard D. Warry, Andrew Flynn, Robin J. Martelli, Francesca Gosling, Rebecca J. Davies, Robert H. La Ragione, Roberto M. Gough, Kevin C. |
| author_facet | Naqid, Ibrahim A. Owen, Jonathan P. Maddison, Ben C. Spiliotopoulos, Anastasios Emes, Richard D. Warry, Andrew Flynn, Robin J. Martelli, Francesca Gosling, Rebecca J. Davies, Robert H. La Ragione, Roberto M. Gough, Kevin C. |
| author_sort | Naqid, Ibrahim A. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Serological surveillance and vaccination are important strategies for controlling infectious diseases of food production animals. However, the compatibility of these strategies is limited by a lack of assays capable of di erentiating infected from vaccinated animals (DIVA tests) for established killed or attenuated vaccines. Here, we used next generation phage-display (NGPD) and a 2-proportion Z score analysis to identify peptides that were preferentially bound by IgY from chickens infected with Salmonella Typhimurium or S. Enteritidis compared to IgY from vaccinates, for both an attenuated and an inactivated commercial vaccine. Peptides that were highly enriched against IgY from at least 4 out of 10 infected chickens were selected: 18 and 12 peptides for the killed and attenuated vaccines, respectively. The ten most discriminatory peptides for each vaccine were identi ed in an ELISA using a training set of IgY samples. These peptides were then used in multi-peptide assays that, when analysing a wider set of samples from infected and vaccinated animals, diagnosed infection with 100% sensitivity and speci city. The data describes a method for the development of DIVA assays for conventional attenuated and killed vaccines. |
| first_indexed | 2025-11-14T19:35:07Z |
| format | Article |
| id | nottingham-38514 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:35:07Z |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-385142020-05-04T18:07:15Z https://eprints.nottingham.ac.uk/38514/ Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals Naqid, Ibrahim A. Owen, Jonathan P. Maddison, Ben C. Spiliotopoulos, Anastasios Emes, Richard D. Warry, Andrew Flynn, Robin J. Martelli, Francesca Gosling, Rebecca J. Davies, Robert H. La Ragione, Roberto M. Gough, Kevin C. Serological surveillance and vaccination are important strategies for controlling infectious diseases of food production animals. However, the compatibility of these strategies is limited by a lack of assays capable of di erentiating infected from vaccinated animals (DIVA tests) for established killed or attenuated vaccines. Here, we used next generation phage-display (NGPD) and a 2-proportion Z score analysis to identify peptides that were preferentially bound by IgY from chickens infected with Salmonella Typhimurium or S. Enteritidis compared to IgY from vaccinates, for both an attenuated and an inactivated commercial vaccine. Peptides that were highly enriched against IgY from at least 4 out of 10 infected chickens were selected: 18 and 12 peptides for the killed and attenuated vaccines, respectively. The ten most discriminatory peptides for each vaccine were identi ed in an ELISA using a training set of IgY samples. These peptides were then used in multi-peptide assays that, when analysing a wider set of samples from infected and vaccinated animals, diagnosed infection with 100% sensitivity and speci city. The data describes a method for the development of DIVA assays for conventional attenuated and killed vaccines. Nature Publishing Group 2016-08-11 Article PeerReviewed Naqid, Ibrahim A., Owen, Jonathan P., Maddison, Ben C., Spiliotopoulos, Anastasios, Emes, Richard D., Warry, Andrew, Flynn, Robin J., Martelli, Francesca, Gosling, Rebecca J., Davies, Robert H., La Ragione, Roberto M. and Gough, Kevin C. (2016) Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals. Scientific Reports, 6 . 31186/1-31186/7. ISSN 2045-2322 http://www.nature.com/articles/srep31186 doi:10.1038/srep31186 doi:10.1038/srep31186 |
| spellingShingle | Naqid, Ibrahim A. Owen, Jonathan P. Maddison, Ben C. Spiliotopoulos, Anastasios Emes, Richard D. Warry, Andrew Flynn, Robin J. Martelli, Francesca Gosling, Rebecca J. Davies, Robert H. La Ragione, Roberto M. Gough, Kevin C. Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals |
| title | Mapping B-cell responses to Salmonella enterica serovars
Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals |
| title_full | Mapping B-cell responses to Salmonella enterica serovars
Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals |
| title_fullStr | Mapping B-cell responses to Salmonella enterica serovars
Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals |
| title_full_unstemmed | Mapping B-cell responses to Salmonella enterica serovars
Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals |
| title_short | Mapping B-cell responses to Salmonella enterica serovars
Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals |
| title_sort | mapping b-cell responses to salmonella enterica serovars
typhimurium and enteritidis in chickens for the discrimination of infected from vaccinated animals |
| url | https://eprints.nottingham.ac.uk/38514/ https://eprints.nottingham.ac.uk/38514/ https://eprints.nottingham.ac.uk/38514/ |