Evolving technologies for growing, imaging and analyzing 3D root system architecture of crop plants

A plant's ability to maintain or improve its yield under limiting conditions, such as nutrient deficiency or drought, can be strongly influenced by root system architecture (RSA), the three-dimensional distribution of the different root types in the soil. The ability to image, track and quantif...

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Main Authors: Piñeros, Miguel A., Larson, Brandon G., Shaff, Jon E., Schneider, David J., Falcão, Alexandre Xavier, Yuan, Lixing, Clark, Randy T., Craft, Eric J., Davis, Tyler W., Pradier, Pierre-Luc, Shaw, Nathanael M., Assaranurak, Ithipong, McCouch, Susan R., Sturrock, Craig, Bennett, Malcolm J., Kochian, Leon V.
Format: Article
Published: Wiley 2016
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Online Access:https://eprints.nottingham.ac.uk/35708/
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author Piñeros, Miguel A.
Larson, Brandon G.
Shaff, Jon E.
Schneider, David J.
Falcão, Alexandre Xavier
Yuan, Lixing
Clark, Randy T.
Craft, Eric J.
Davis, Tyler W.
Pradier, Pierre-Luc
Shaw, Nathanael M.
Assaranurak, Ithipong
McCouch, Susan R.
Sturrock, Craig
Bennett, Malcolm J.
Kochian, Leon V.
author_facet Piñeros, Miguel A.
Larson, Brandon G.
Shaff, Jon E.
Schneider, David J.
Falcão, Alexandre Xavier
Yuan, Lixing
Clark, Randy T.
Craft, Eric J.
Davis, Tyler W.
Pradier, Pierre-Luc
Shaw, Nathanael M.
Assaranurak, Ithipong
McCouch, Susan R.
Sturrock, Craig
Bennett, Malcolm J.
Kochian, Leon V.
author_sort Piñeros, Miguel A.
building Nottingham Research Data Repository
collection Online Access
description A plant's ability to maintain or improve its yield under limiting conditions, such as nutrient deficiency or drought, can be strongly influenced by root system architecture (RSA), the three-dimensional distribution of the different root types in the soil. The ability to image, track and quantify these root system attributes in a dynamic fashion is a useful tool in assessing desirable genetic and physiological root traits. Recent advances in imaging technology and phenotyping software have resulted in substantive progress in describing and quantifying RSA. We have designed a hydroponic growth system which retains the three-dimensional RSA of the plant root system, while allowing for aeration, solution replenishment and the imposition of nutrient treatments, as well as high-quality imaging of the root system. The simplicity and flexibility of the system allows for modifications tailored to the RSA of different crop species and improved throughput. This paper details the recent improvements and innovations in our root growth and imaging system which allows for greater image sensitivity (detection of fine roots and other root details), higher efficiency, and a broad array of growing conditions for plants that more closely mimic those found under field conditions.
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spelling nottingham-357082020-05-04T17:41:26Z https://eprints.nottingham.ac.uk/35708/ Evolving technologies for growing, imaging and analyzing 3D root system architecture of crop plants Piñeros, Miguel A. Larson, Brandon G. Shaff, Jon E. Schneider, David J. Falcão, Alexandre Xavier Yuan, Lixing Clark, Randy T. Craft, Eric J. Davis, Tyler W. Pradier, Pierre-Luc Shaw, Nathanael M. Assaranurak, Ithipong McCouch, Susan R. Sturrock, Craig Bennett, Malcolm J. Kochian, Leon V. A plant's ability to maintain or improve its yield under limiting conditions, such as nutrient deficiency or drought, can be strongly influenced by root system architecture (RSA), the three-dimensional distribution of the different root types in the soil. The ability to image, track and quantify these root system attributes in a dynamic fashion is a useful tool in assessing desirable genetic and physiological root traits. Recent advances in imaging technology and phenotyping software have resulted in substantive progress in describing and quantifying RSA. We have designed a hydroponic growth system which retains the three-dimensional RSA of the plant root system, while allowing for aeration, solution replenishment and the imposition of nutrient treatments, as well as high-quality imaging of the root system. The simplicity and flexibility of the system allows for modifications tailored to the RSA of different crop species and improved throughput. This paper details the recent improvements and innovations in our root growth and imaging system which allows for greater image sensitivity (detection of fine roots and other root details), higher efficiency, and a broad array of growing conditions for plants that more closely mimic those found under field conditions. Wiley 2016-03-17 Article PeerReviewed Piñeros, Miguel A., Larson, Brandon G., Shaff, Jon E., Schneider, David J., Falcão, Alexandre Xavier, Yuan, Lixing, Clark, Randy T., Craft, Eric J., Davis, Tyler W., Pradier, Pierre-Luc, Shaw, Nathanael M., Assaranurak, Ithipong, McCouch, Susan R., Sturrock, Craig, Bennett, Malcolm J. and Kochian, Leon V. (2016) Evolving technologies for growing, imaging and analyzing 3D root system architecture of crop plants. Journal of Integrative Plant Biology, 58 (3). pp. 230-241. ISSN 1672-9072 Abiotic stress; digital root phenotyping; mineral nutrition; root system architecture http://onlinelibrary.wiley.com/doi/10.1111/jipb.12456/abstract doi:10.1111/jipb.12456 doi:10.1111/jipb.12456
spellingShingle Abiotic stress; digital root phenotyping; mineral nutrition; root system architecture
Piñeros, Miguel A.
Larson, Brandon G.
Shaff, Jon E.
Schneider, David J.
Falcão, Alexandre Xavier
Yuan, Lixing
Clark, Randy T.
Craft, Eric J.
Davis, Tyler W.
Pradier, Pierre-Luc
Shaw, Nathanael M.
Assaranurak, Ithipong
McCouch, Susan R.
Sturrock, Craig
Bennett, Malcolm J.
Kochian, Leon V.
Evolving technologies for growing, imaging and analyzing 3D root system architecture of crop plants
title Evolving technologies for growing, imaging and analyzing 3D root system architecture of crop plants
title_full Evolving technologies for growing, imaging and analyzing 3D root system architecture of crop plants
title_fullStr Evolving technologies for growing, imaging and analyzing 3D root system architecture of crop plants
title_full_unstemmed Evolving technologies for growing, imaging and analyzing 3D root system architecture of crop plants
title_short Evolving technologies for growing, imaging and analyzing 3D root system architecture of crop plants
title_sort evolving technologies for growing, imaging and analyzing 3d root system architecture of crop plants
topic Abiotic stress; digital root phenotyping; mineral nutrition; root system architecture
url https://eprints.nottingham.ac.uk/35708/
https://eprints.nottingham.ac.uk/35708/
https://eprints.nottingham.ac.uk/35708/