Application of BRET to monitor ligand binding to GPCRs
Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein–coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligand...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
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Nature Publishing Group
2015
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| Online Access: | https://eprints.nottingham.ac.uk/35176/ |
| _version_ | 1848795021181976576 |
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| author | Stoddart, Leigh A. Johnstone, Elizabeth K.M. Wheal, Amanda J. Goulding, Joelle Robers, Matthew B. Machleidt, Thomas Wood, Keith V. Hill, Stephen J. Pfleger, Kevin D.G. |
| author_facet | Stoddart, Leigh A. Johnstone, Elizabeth K.M. Wheal, Amanda J. Goulding, Joelle Robers, Matthew B. Machleidt, Thomas Wood, Keith V. Hill, Stephen J. Pfleger, Kevin D.G. |
| author_sort | Stoddart, Leigh A. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein–coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs. |
| first_indexed | 2025-11-14T19:25:27Z |
| format | Article |
| id | nottingham-35176 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:25:27Z |
| publishDate | 2015 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-351762020-05-04T17:07:32Z https://eprints.nottingham.ac.uk/35176/ Application of BRET to monitor ligand binding to GPCRs Stoddart, Leigh A. Johnstone, Elizabeth K.M. Wheal, Amanda J. Goulding, Joelle Robers, Matthew B. Machleidt, Thomas Wood, Keith V. Hill, Stephen J. Pfleger, Kevin D.G. Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein–coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs. Nature Publishing Group 2015-06-01 Article PeerReviewed Stoddart, Leigh A., Johnstone, Elizabeth K.M., Wheal, Amanda J., Goulding, Joelle, Robers, Matthew B., Machleidt, Thomas, Wood, Keith V., Hill, Stephen J. and Pfleger, Kevin D.G. (2015) Application of BRET to monitor ligand binding to GPCRs. Nature Methods, 12 . pp. 661-663. ISSN 1548-7105 cell signalling drug development optical spectroscopy http://www.nature.com/nmeth/journal/v12/n7/full/nmeth.3398.html doi:10.1038/nmeth.3398 doi:10.1038/nmeth.3398 |
| spellingShingle | cell signalling drug development optical spectroscopy Stoddart, Leigh A. Johnstone, Elizabeth K.M. Wheal, Amanda J. Goulding, Joelle Robers, Matthew B. Machleidt, Thomas Wood, Keith V. Hill, Stephen J. Pfleger, Kevin D.G. Application of BRET to monitor ligand binding to GPCRs |
| title | Application of BRET to monitor ligand binding to GPCRs |
| title_full | Application of BRET to monitor ligand binding to GPCRs |
| title_fullStr | Application of BRET to monitor ligand binding to GPCRs |
| title_full_unstemmed | Application of BRET to monitor ligand binding to GPCRs |
| title_short | Application of BRET to monitor ligand binding to GPCRs |
| title_sort | application of bret to monitor ligand binding to gpcrs |
| topic | cell signalling drug development optical spectroscopy |
| url | https://eprints.nottingham.ac.uk/35176/ https://eprints.nottingham.ac.uk/35176/ https://eprints.nottingham.ac.uk/35176/ |