Use of a new proximity assay (NanoBRET) to investigate the ligand binding characteristics of three fluorescent ligands to the human β1-adrenoceptor expressed in HEK-293 cells

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Use of a new proximity assay (NanoBRET) to investigate the ligand binding characteristics of three fluorescent ligands to the human β1-adrenoceptor expressed in HEK-293 cells

Previous research has indicated that allosteric interactions across the dimer interface of β1-adrenoceptors may be responsible for a secondary low affinity binding conformation. Here we have investigated the potential for probe dependence, in the determination of antagonist pKi values at the human β...

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Bibliographic Details
Main Authors:	Soave, Mark, Stoddart, Leigh A., Brown, Alastair, Woolard, Jeanette, Hill, Stephen J.
Format:	Article
Published:	Wiley 2016
Subjects:	Bioluminescence energy transfer; Ligand-binding; 3-adrenoceptors; Probe dependence
Online Access:	https://eprints.nottingham.ac.uk/34685/

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