Use of a new proximity assay (NanoBRET) to investigate the ligand binding characteristics of three fluorescent ligands to the human β1-adrenoceptor expressed in HEK-293 cells
Previous research has indicated that allosteric interactions across the dimer interface of β1-adrenoceptors may be responsible for a secondary low affinity binding conformation. Here we have investigated the potential for probe dependence, in the determination of antagonist pKi values at the human β...
| Main Authors: | , , , , |
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| Format: | Article |
| Published: |
Wiley
2016
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| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/34685/ |