Flexible and rapid construction of viral chimeras applied to Hepatitis C Virus

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Flexible and rapid construction of viral chimeras applied to Hepatitis C Virus

A novel and broadly applicable strategy combining site directed mutagenesis and DNA assembly for constructing seamless viral chimeras is described using Hepatitis C Virus as an exemplar. Full-length HCV genomic cloning cassettes, which contained flexibly situated restriction endonuclease sites, were...

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Bibliographic Details
Main Authors:	McClure, Patrick C., Urbanowicz, Richard A., King, Barnabas J., Cano-Crespo, Sara, Tarr, Alexander W., Ball, Jonathan K.
Format:	Article
Published:	Microbiology Society 2016
Online Access:	https://eprints.nottingham.ac.uk/34493/

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