Flexible and rapid construction of viral chimeras applied to Hepatitis C Virus
A novel and broadly applicable strategy combining site directed mutagenesis and DNA assembly for constructing seamless viral chimeras is described using Hepatitis C Virus as an exemplar. Full-length HCV genomic cloning cassettes, which contained flexibly situated restriction endonuclease sites, were...
| Main Authors: | , , , , , |
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| Format: | Article |
| Published: |
Microbiology Society
2016
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| Online Access: | https://eprints.nottingham.ac.uk/34493/ |