Total loss of MHC class I is an independent indicator of good prognosis in breast cancer

Tumours can be recognised by CTL and NK cells. CTL recognition depends on expression of MHC Class I loaded with peptides from tumour antigens. In contrast, loss of MHC Class I results in NK activation. In our study a large set of samples from patients with primary operable invasive breast cancer was...

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Main Authors: Madjd, Zahra, Spendlove, Ian, Pinder, Sarah E., Ellis, Ian O., Durrant, Lindy G.
Format: Article
Published: Wiley 2005
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Online Access:https://eprints.nottingham.ac.uk/3145/
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author Madjd, Zahra
Spendlove, Ian
Pinder, Sarah E.
Ellis, Ian O.
Durrant, Lindy G.
author_facet Madjd, Zahra
Spendlove, Ian
Pinder, Sarah E.
Ellis, Ian O.
Durrant, Lindy G.
author_sort Madjd, Zahra
building Nottingham Research Data Repository
collection Online Access
description Tumours can be recognised by CTL and NK cells. CTL recognition depends on expression of MHC Class I loaded with peptides from tumour antigens. In contrast, loss of MHC Class I results in NK activation. In our study a large set of samples from patients with primary operable invasive breast cancer was evaluated for the expression of MHC Class I heavy and light by immunohistochemical staining of 439 breast carcinomas in a tissue microarray. Forty-seven percent (206 of 439) of breast carcinomas were considered negative for HLA Class I heavy chain (HC10), whereas lack of anti-β2m-antibody staining was observed in 39% (167 of 424) of tumours, with only 3% of the β2m-negative tumours expressing detectable HLA Class I heavy chain. Correlation with patient outcome showed direct relationship between patient survival and HLA-negative phenotype (log rank = 0.004). A positive relationship was found between the intensity of expression of MHC Class I light and heavy chains expression and histological grade of invasive tumour (p < 0.001) and Nottingham Prognostic Index (p < 0.001). To investigate whether HLA Class I heavy and light chains expression had independent prognostic significance, Cox multivariate regression analysis, including the parameters of tumour size, lymph node stage, grade and intensity of HC10 and anti-β2m staining, was carried out. In our analysis, lymph node stage (p < 0.001), tumour grade (p = 0.005) and intensity of MHC Class I light and heavy chains expression were shown to be independent prognostic factors predictive of overall survival (p-values HC10 = 0.047 and β2m = 0.018).
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spelling nottingham-31452020-05-04T16:26:12Z https://eprints.nottingham.ac.uk/3145/ Total loss of MHC class I is an independent indicator of good prognosis in breast cancer Madjd, Zahra Spendlove, Ian Pinder, Sarah E. Ellis, Ian O. Durrant, Lindy G. Tumours can be recognised by CTL and NK cells. CTL recognition depends on expression of MHC Class I loaded with peptides from tumour antigens. In contrast, loss of MHC Class I results in NK activation. In our study a large set of samples from patients with primary operable invasive breast cancer was evaluated for the expression of MHC Class I heavy and light by immunohistochemical staining of 439 breast carcinomas in a tissue microarray. Forty-seven percent (206 of 439) of breast carcinomas were considered negative for HLA Class I heavy chain (HC10), whereas lack of anti-β2m-antibody staining was observed in 39% (167 of 424) of tumours, with only 3% of the β2m-negative tumours expressing detectable HLA Class I heavy chain. Correlation with patient outcome showed direct relationship between patient survival and HLA-negative phenotype (log rank = 0.004). A positive relationship was found between the intensity of expression of MHC Class I light and heavy chains expression and histological grade of invasive tumour (p < 0.001) and Nottingham Prognostic Index (p < 0.001). To investigate whether HLA Class I heavy and light chains expression had independent prognostic significance, Cox multivariate regression analysis, including the parameters of tumour size, lymph node stage, grade and intensity of HC10 and anti-β2m staining, was carried out. In our analysis, lymph node stage (p < 0.001), tumour grade (p = 0.005) and intensity of MHC Class I light and heavy chains expression were shown to be independent prognostic factors predictive of overall survival (p-values HC10 = 0.047 and β2m = 0.018). Wiley 2005-11-01 Article PeerReviewed Madjd, Zahra, Spendlove, Ian, Pinder, Sarah E., Ellis, Ian O. and Durrant, Lindy G. (2005) Total loss of MHC class I is an independent indicator of good prognosis in breast cancer. International Journal of Cancer, 117 (2). pp. 248-255. ISSN 0020-7136 Breast tissue microarray HC10 Prognostic factor MHC Class I Immunohistochemistry http://onlinelibrary.wiley.com/doi/10.1002/ijc.21163/abstract;jsessionid=0F2D2BA7158A645CEAA0FEC990F1DD0E.f02t04 doi:10.1002/ijc.21163 doi:10.1002/ijc.21163
spellingShingle Breast tissue microarray
HC10
Prognostic factor
MHC Class I
Immunohistochemistry
Madjd, Zahra
Spendlove, Ian
Pinder, Sarah E.
Ellis, Ian O.
Durrant, Lindy G.
Total loss of MHC class I is an independent indicator of good prognosis in breast cancer
title Total loss of MHC class I is an independent indicator of good prognosis in breast cancer
title_full Total loss of MHC class I is an independent indicator of good prognosis in breast cancer
title_fullStr Total loss of MHC class I is an independent indicator of good prognosis in breast cancer
title_full_unstemmed Total loss of MHC class I is an independent indicator of good prognosis in breast cancer
title_short Total loss of MHC class I is an independent indicator of good prognosis in breast cancer
title_sort total loss of mhc class i is an independent indicator of good prognosis in breast cancer
topic Breast tissue microarray
HC10
Prognostic factor
MHC Class I
Immunohistochemistry
url https://eprints.nottingham.ac.uk/3145/
https://eprints.nottingham.ac.uk/3145/
https://eprints.nottingham.ac.uk/3145/