Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair
Using the ASKA (A Complete Set of E. coli K-12 ORF Archive) library for genome-wide screening of E. coli proteins we identified that expression of ygaQ and rpmG promotemitomycin C resistance (MMCR). YgaQ mediated MMCR was independent of homologous recombination involving RecA or RuvABC, but re...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Published: |
Portland Press:
2015
|
| Online Access: | https://eprints.nottingham.ac.uk/31311/ |
| _version_ | 1848794174473633792 |
|---|---|
| author | Bolt, Edward L. Jenkins, Tabitha Russo, Valeria Moreira Ahmed, Sharlene Cavey, James Cass, Simon |
| author_facet | Bolt, Edward L. Jenkins, Tabitha Russo, Valeria Moreira Ahmed, Sharlene Cavey, James Cass, Simon |
| author_sort | Bolt, Edward L. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Using the ASKA (A Complete Set of E. coli K-12 ORF Archive) library for genome-wide screening of E. coli proteins we identified that expression of ygaQ and rpmG promotemitomycin C resistance (MMCR). YgaQ mediated MMCR was independent of homologous recombination involving RecA or RuvABC, but required UvrD. YgaQ is an uncharacterized protein homologous to a-amylases that we identified to have nuclease activity directed to single stranded DNA of 5’ flaps. Nuclease activity was inactivated by mutation of two amino acid motifs, which also abolished MMCR. RpmG is frequently annotated as a bacterial ribosomal protein, although forms an operon with MutM glycosylase and a putative deubiquitinating enzyme, YicR. RpmG associated MMCR was dependent on MutM. MMCR from RpmG resembles DNA repair phenotypes reported for ‘idiosyncratic ribosomal proteins’ in eukaryotes. |
| first_indexed | 2025-11-14T19:12:00Z |
| format | Article |
| id | nottingham-31311 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:12:00Z |
| publishDate | 2015 |
| publisher | Portland Press: |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-313112020-05-04T17:25:48Z https://eprints.nottingham.ac.uk/31311/ Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair Bolt, Edward L. Jenkins, Tabitha Russo, Valeria Moreira Ahmed, Sharlene Cavey, James Cass, Simon Using the ASKA (A Complete Set of E. coli K-12 ORF Archive) library for genome-wide screening of E. coli proteins we identified that expression of ygaQ and rpmG promotemitomycin C resistance (MMCR). YgaQ mediated MMCR was independent of homologous recombination involving RecA or RuvABC, but required UvrD. YgaQ is an uncharacterized protein homologous to a-amylases that we identified to have nuclease activity directed to single stranded DNA of 5’ flaps. Nuclease activity was inactivated by mutation of two amino acid motifs, which also abolished MMCR. RpmG is frequently annotated as a bacterial ribosomal protein, although forms an operon with MutM glycosylase and a putative deubiquitinating enzyme, YicR. RpmG associated MMCR was dependent on MutM. MMCR from RpmG resembles DNA repair phenotypes reported for ‘idiosyncratic ribosomal proteins’ in eukaryotes. Portland Press: 2015-12-24 Article PeerReviewed Bolt, Edward L., Jenkins, Tabitha, Russo, Valeria Moreira, Ahmed, Sharlene, Cavey, James and Cass, Simon (2015) Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair. Bioscience Reports . pp. 1-9. ISSN 1573-4935 http://www.bioscirep.org/content/early/2015/12/21/BSR20150249 doi:10.1042/BSR20150249 doi:10.1042/BSR20150249 |
| spellingShingle | Bolt, Edward L. Jenkins, Tabitha Russo, Valeria Moreira Ahmed, Sharlene Cavey, James Cass, Simon Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair |
| title | Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair |
| title_full | Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair |
| title_fullStr | Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair |
| title_full_unstemmed | Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair |
| title_short | Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair |
| title_sort | identification of escherichia coli ygaq and rpmg as novel mitomycin c resistance factors implicated in dna repair |
| url | https://eprints.nottingham.ac.uk/31311/ https://eprints.nottingham.ac.uk/31311/ https://eprints.nottingham.ac.uk/31311/ |