Study of intragastric structuring ability of sodium alginate based o/w emulsions under in vitro physiological pre-absorptive digestion conditions

In the present work, the intragastric structuring ability of o/w emulsions either stabilised (1–4%, w/w of sodium alginate (SA)) or structured with sheared ionic gel (1–3%, w/w of SA crosslinked with Ca2+) in the absence (saliva and gastric phases constituted of deionised water) or presence of in vi...

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Main Authors: Soukoulis, Christos, Fisk, Ian D., Bohn, Torsten, Hoffmann, Lucien
Format: Article
Published: Elsevier 2016
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Online Access:https://eprints.nottingham.ac.uk/31230/
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author Soukoulis, Christos
Fisk, Ian D.
Bohn, Torsten
Hoffmann, Lucien
author_facet Soukoulis, Christos
Fisk, Ian D.
Bohn, Torsten
Hoffmann, Lucien
author_sort Soukoulis, Christos
building Nottingham Research Data Repository
collection Online Access
description In the present work, the intragastric structuring ability of o/w emulsions either stabilised (1–4%, w/w of sodium alginate (SA)) or structured with sheared ionic gel (1–3%, w/w of SA crosslinked with Ca2+) in the absence (saliva and gastric phases constituted of deionised water) or presence of in vitro pre-absorptive conditions (physiological simulated saliva and gastric fluids) was investigated. Visualisation of the morphological aspects of the gastric chymes, in the absence of multivalent counterions, demonstrated that SA stabilised systems underwent a remarkable swelling in the pH range of 2–3, whilst at the same pH range, ionic SA gel structured systems maintained their major structure configuration. When the aforementioned systems were exposed to physiological intragastric fluids, a reduction of the length and the hydrodynamic volume of the alginate fibres was detected regardless the structuring approach. On their exposure to physiological intragastric conditions (pH = 2), SA stabilised emulsions underwent sol–gel transition achieving a ca. 3- to 4-order increase of storage modulus (at 1 Hz). In the case of ionic sheared gel structured emulsions, exposure to physiological intragastric fluids resulted in a 10-fold reduction ability of their acid structuring ability, most likely due to the dialysis of egg-box dimer conformations by monovalent cations and protons and the sterical hindering of hydrogen bonding of MM and GG sequences under acidic conditions. Using of non-physiological simulated intragastric fluids was associated with overestimated structuring performance of SA regardless its physical state.
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spelling nottingham-312302020-05-04T17:45:52Z https://eprints.nottingham.ac.uk/31230/ Study of intragastric structuring ability of sodium alginate based o/w emulsions under in vitro physiological pre-absorptive digestion conditions Soukoulis, Christos Fisk, Ian D. Bohn, Torsten Hoffmann, Lucien In the present work, the intragastric structuring ability of o/w emulsions either stabilised (1–4%, w/w of sodium alginate (SA)) or structured with sheared ionic gel (1–3%, w/w of SA crosslinked with Ca2+) in the absence (saliva and gastric phases constituted of deionised water) or presence of in vitro pre-absorptive conditions (physiological simulated saliva and gastric fluids) was investigated. Visualisation of the morphological aspects of the gastric chymes, in the absence of multivalent counterions, demonstrated that SA stabilised systems underwent a remarkable swelling in the pH range of 2–3, whilst at the same pH range, ionic SA gel structured systems maintained their major structure configuration. When the aforementioned systems were exposed to physiological intragastric fluids, a reduction of the length and the hydrodynamic volume of the alginate fibres was detected regardless the structuring approach. On their exposure to physiological intragastric conditions (pH = 2), SA stabilised emulsions underwent sol–gel transition achieving a ca. 3- to 4-order increase of storage modulus (at 1 Hz). In the case of ionic sheared gel structured emulsions, exposure to physiological intragastric fluids resulted in a 10-fold reduction ability of their acid structuring ability, most likely due to the dialysis of egg-box dimer conformations by monovalent cations and protons and the sterical hindering of hydrogen bonding of MM and GG sequences under acidic conditions. Using of non-physiological simulated intragastric fluids was associated with overestimated structuring performance of SA regardless its physical state. Elsevier 2016-04-20 Article PeerReviewed Soukoulis, Christos, Fisk, Ian D., Bohn, Torsten and Hoffmann, Lucien (2016) Study of intragastric structuring ability of sodium alginate based o/w emulsions under in vitro physiological pre-absorptive digestion conditions. Carbohydrate Polymers, 140 . pp. 26-34. ISSN 1879-1344 Acid Gelation Ionotropic Gelation Rheology Gastric Fluids Pre-Absorptive Digestion Conditions http://www.sciencedirect.com/science/article/pii/S0144861715011996 doi:10.1016/j.carbpol.2015.12.021 doi:10.1016/j.carbpol.2015.12.021
spellingShingle Acid Gelation
Ionotropic Gelation
Rheology
Gastric Fluids
Pre-Absorptive Digestion Conditions
Soukoulis, Christos
Fisk, Ian D.
Bohn, Torsten
Hoffmann, Lucien
Study of intragastric structuring ability of sodium alginate based o/w emulsions under in vitro physiological pre-absorptive digestion conditions
title Study of intragastric structuring ability of sodium alginate based o/w emulsions under in vitro physiological pre-absorptive digestion conditions
title_full Study of intragastric structuring ability of sodium alginate based o/w emulsions under in vitro physiological pre-absorptive digestion conditions
title_fullStr Study of intragastric structuring ability of sodium alginate based o/w emulsions under in vitro physiological pre-absorptive digestion conditions
title_full_unstemmed Study of intragastric structuring ability of sodium alginate based o/w emulsions under in vitro physiological pre-absorptive digestion conditions
title_short Study of intragastric structuring ability of sodium alginate based o/w emulsions under in vitro physiological pre-absorptive digestion conditions
title_sort study of intragastric structuring ability of sodium alginate based o/w emulsions under in vitro physiological pre-absorptive digestion conditions
topic Acid Gelation
Ionotropic Gelation
Rheology
Gastric Fluids
Pre-Absorptive Digestion Conditions
url https://eprints.nottingham.ac.uk/31230/
https://eprints.nottingham.ac.uk/31230/
https://eprints.nottingham.ac.uk/31230/