Engineering a reagentless biosensor for single-stranded DNA
to measure real-time helicase activity in Bacillus

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Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus

Single-stranded DNA-binding protein(SSB)is a well characterized ubiquitous and essential bacterial protein involved in almost all aspects of DNA metabolism. Using the Bacillus subtilis SSB we have generated areagentless SSB biosensor that can be used as a helicase probe in B. subtilis and closely r...

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Bibliographic Details
Main Authors:	Green, Matthew, Gilhooly, Neville S., Abedeen, Shahriar, Scott, David J., Dillingham, Mark S., Soultanas, Panos
Format:	Article
Language:	English
Published:	Elsevier 2014
Subjects:	SSB Helicases Fluorescence DNA unwinding Bacillus subtilis Firmicutes
Online Access:	https://eprints.nottingham.ac.uk/31164/

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