Engineering a reagentless biosensor for single-stranded DNA to measure real-time helicase activity in Bacillus
Single-stranded DNA-binding protein(SSB)is a well characterized ubiquitous and essential bacterial protein involved in almost all aspects of DNA metabolism. Using the Bacillus subtilis SSB we have generated areagentless SSB biosensor that can be used as a helicase probe in B. subtilis and closely r...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014
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| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/31164/ |