Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System
The PIMMS (Pragmatic Insertional Mutation Mapping System) pipeline has beendeveloped for simple conditionally essential genome discovery experiments in bacteria.Capable of using raw sequence data files alongside a FASTA sequence of thereference genome and GFF file, PIMMS will generate a tabulated ou...
| Main Authors: | , , , |
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| Format: | Article |
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Frontiers
2015
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| Online Access: | https://eprints.nottingham.ac.uk/31135/ |
| _version_ | 1848794133730164736 |
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| author | Blanchard, Adam M. Leigh, James A. Egan, Sharon A. Emes, Richard D. |
| author_facet | Blanchard, Adam M. Leigh, James A. Egan, Sharon A. Emes, Richard D. |
| author_sort | Blanchard, Adam M. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The PIMMS (Pragmatic Insertional Mutation Mapping System) pipeline has beendeveloped for simple conditionally essential genome discovery experiments in bacteria.Capable of using raw sequence data files alongside a FASTA sequence of thereference genome and GFF file, PIMMS will generate a tabulated output of each codingsequence with corresponding mapped insertions accompanied with normalized resultsenabling streamlined analysis. This allows for a quick assay of the genome to identifyconditionally essential genes on a standar d desktop computer prioritizing results forfurther investigation. |
| first_indexed | 2025-11-14T19:11:21Z |
| format | Article |
| id | nottingham-31135 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:11:21Z |
| publishDate | 2015 |
| publisher | Frontiers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-311352020-05-04T17:06:57Z https://eprints.nottingham.ac.uk/31135/ Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System Blanchard, Adam M. Leigh, James A. Egan, Sharon A. Emes, Richard D. The PIMMS (Pragmatic Insertional Mutation Mapping System) pipeline has beendeveloped for simple conditionally essential genome discovery experiments in bacteria.Capable of using raw sequence data files alongside a FASTA sequence of thereference genome and GFF file, PIMMS will generate a tabulated output of each codingsequence with corresponding mapped insertions accompanied with normalized resultsenabling streamlined analysis. This allows for a quick assay of the genome to identifyconditionally essential genes on a standar d desktop computer prioritizing results forfurther investigation. Frontiers 2015-04-09 Article PeerReviewed Blanchard, Adam M., Leigh, James A., Egan, Sharon A. and Emes, Richard D. (2015) Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System. Frontiers in Genetics, 6 . e139/1-e139/8. ISSN 1664-8021 http://journal.frontiersin.org/article/10.3389/fgene.2015.00139/abstract doi:10.3389/fgene.2015.00139 doi:10.3389/fgene.2015.00139 |
| spellingShingle | Blanchard, Adam M. Leigh, James A. Egan, Sharon A. Emes, Richard D. Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System |
| title | Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System |
| title_full | Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System |
| title_fullStr | Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System |
| title_full_unstemmed | Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System |
| title_short | Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System |
| title_sort | transposon insertion mapping with pimms, pragmatic insertional mutation mapping system |
| url | https://eprints.nottingham.ac.uk/31135/ https://eprints.nottingham.ac.uk/31135/ https://eprints.nottingham.ac.uk/31135/ |