Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System

The PIMMS (Pragmatic Insertional Mutation Mapping System) pipeline has beendeveloped for simple conditionally essential genome discovery experiments in bacteria.Capable of using raw sequence data files alongside a FASTA sequence of thereference genome and GFF file, PIMMS will generate a tabulated ou...

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Bibliographic Details
Main Authors: Blanchard, Adam M., Leigh, James A., Egan, Sharon A., Emes, Richard D.
Format: Article
Published: Frontiers 2015
Online Access:https://eprints.nottingham.ac.uk/31135/