Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker

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Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker

Most bacteria can only be transformed with circular plasmids, so robust DNA integration methods for these rely upon selection of single-crossover clones followed by counter-selection of double-crossover clones. To overcome the limited availability of heterologous counter-selection markers, here we e...

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Bibliographic Details
Main Authors:	Heap, John T., Ehsaan, Muhammad, Cooksley, Clare M., Ng, Yen-Kuan, Cartman, Stephen T., Winzer, Klaus, Minton, Nigel P.
Format:	Article
Published:	Oxford University Press (OUP) 2012
Online Access:	https://eprints.nottingham.ac.uk/3062/

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