Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker
Most bacteria can only be transformed with circular plasmids, so robust DNA integration methods for these rely upon selection of single-crossover clones followed by counter-selection of double-crossover clones. To overcome the limited availability of heterologous counter-selection markers, here we e...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Oxford University Press (OUP)
2012
|
| Online Access: | https://eprints.nottingham.ac.uk/3062/ |