The use of viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein eGFP
Introduction: The viral 2A sequence has become an attractive alternative to the traditional internal ribosomal entry site (IRES) for simultaneous over-expression of two genes and in combination with recombinant adeno-associated viruses (rAAV) has been used to manipulate gene expression in vitro. Ne...
| Main Authors: | , , , , , |
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| Format: | Article |
| Published: |
Elsevier
2015
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| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/29726/ |