A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants
Activated forms of jasmonic acid (JA) are central signals coordinating plant responses to stresses, yet tools to analyse their spatial and temporal distribution are lacking. Here we describe a JA perception biosensor termed Jas9-VENUS that allows the quantification of dynamic changes in JA distribut...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
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Nature Publishing Group
2015
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| Online Access: | https://eprints.nottingham.ac.uk/29320/ |
| _version_ | 1848793761008582656 |
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| author | Larrieu, Antoine Champion, Antony Legrand, Jonathan Lavenus, Julien Mast, David Brunoud, Geraldine Oh, Jaesung Guyomarc’h, Soazig Pizot, Maxime Farmer, Edward E. Turnbull, Colin Vernoux, Teva Bennett, Malcolm J. Laplaze, Laurent |
| author_facet | Larrieu, Antoine Champion, Antony Legrand, Jonathan Lavenus, Julien Mast, David Brunoud, Geraldine Oh, Jaesung Guyomarc’h, Soazig Pizot, Maxime Farmer, Edward E. Turnbull, Colin Vernoux, Teva Bennett, Malcolm J. Laplaze, Laurent |
| author_sort | Larrieu, Antoine |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Activated forms of jasmonic acid (JA) are central signals coordinating plant responses to stresses, yet tools to analyse their spatial and temporal distribution are lacking. Here we describe a JA perception biosensor termed Jas9-VENUS that allows the quantification of dynamic changes in JA distribution in response to stress with high spatiotemporal sensitivity. We show that Jas9-VENUS abundance is dependent on bioactive JA isoforms, the COI1 co-receptor, a functional Jas motif and proteasome activity. We demonstrate the utility of Jas9-VENUS to analyse responses to JA in planta at a cellular scale, both quantitatively and dynamically. This included using Jas9-VENUS to determine the cotyledon-to-root JA signal velocities on wounding, revealing two distinct phases of JA activity in the root. Our results demonstrate the value of developing quantitative sensors such as Jas9-VENUS to provide high-resolution spatiotemporal data about hormone distribution in response to plant abiotic and biotic stresses. |
| first_indexed | 2025-11-14T19:05:26Z |
| format | Article |
| id | nottingham-29320 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:05:26Z |
| publishDate | 2015 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-293202020-05-04T17:00:44Z https://eprints.nottingham.ac.uk/29320/ A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants Larrieu, Antoine Champion, Antony Legrand, Jonathan Lavenus, Julien Mast, David Brunoud, Geraldine Oh, Jaesung Guyomarc’h, Soazig Pizot, Maxime Farmer, Edward E. Turnbull, Colin Vernoux, Teva Bennett, Malcolm J. Laplaze, Laurent Activated forms of jasmonic acid (JA) are central signals coordinating plant responses to stresses, yet tools to analyse their spatial and temporal distribution are lacking. Here we describe a JA perception biosensor termed Jas9-VENUS that allows the quantification of dynamic changes in JA distribution in response to stress with high spatiotemporal sensitivity. We show that Jas9-VENUS abundance is dependent on bioactive JA isoforms, the COI1 co-receptor, a functional Jas motif and proteasome activity. We demonstrate the utility of Jas9-VENUS to analyse responses to JA in planta at a cellular scale, both quantitatively and dynamically. This included using Jas9-VENUS to determine the cotyledon-to-root JA signal velocities on wounding, revealing two distinct phases of JA activity in the root. Our results demonstrate the value of developing quantitative sensors such as Jas9-VENUS to provide high-resolution spatiotemporal data about hormone distribution in response to plant abiotic and biotic stresses. Nature Publishing Group 2015-01-16 Article PeerReviewed Larrieu, Antoine, Champion, Antony, Legrand, Jonathan, Lavenus, Julien, Mast, David, Brunoud, Geraldine, Oh, Jaesung, Guyomarc’h, Soazig, Pizot, Maxime, Farmer, Edward E., Turnbull, Colin, Vernoux, Teva, Bennett, Malcolm J. and Laplaze, Laurent (2015) A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants. Nature Communications, 6 (6043). pp. 1-8. ISSN 2041-1723 Arabidopsis jasmonate jasminic acid biosensor signalling http://www.nature.com/ncomms/2015/150116/ncomms7043/full/ncomms7043.html doi:10.1038/ncomms7043 doi:10.1038/ncomms7043 |
| spellingShingle | Arabidopsis jasmonate jasminic acid biosensor signalling Larrieu, Antoine Champion, Antony Legrand, Jonathan Lavenus, Julien Mast, David Brunoud, Geraldine Oh, Jaesung Guyomarc’h, Soazig Pizot, Maxime Farmer, Edward E. Turnbull, Colin Vernoux, Teva Bennett, Malcolm J. Laplaze, Laurent A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants |
| title | A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants |
| title_full | A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants |
| title_fullStr | A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants |
| title_full_unstemmed | A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants |
| title_short | A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants |
| title_sort | fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants |
| topic | Arabidopsis jasmonate jasminic acid biosensor signalling |
| url | https://eprints.nottingham.ac.uk/29320/ https://eprints.nottingham.ac.uk/29320/ https://eprints.nottingham.ac.uk/29320/ |