The enzyme activities of Caf1 and Ccr4 are both required for deadenylation by the human Ccr4-Not nuclease module
In eukaryotic cells, the shortening and removal of the poly(A) tail (deadenylation) of cytoplasmic mRNA is a key event in regulated mRNA degradation. A major enzyme involved in deadenylation is the Ccr4-Not deadenylase complex, which can be recruited to its target mRNA by RNA-binding proteins or the...
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Portland Press
2015
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| Online Access: | https://eprints.nottingham.ac.uk/28955/ |
| _version_ | 1848793681229774848 |
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| author | Maryati, Marayti Airhihen, Blessing Winkler, G. Sebastiaan |
| author_facet | Maryati, Marayti Airhihen, Blessing Winkler, G. Sebastiaan |
| author_sort | Maryati, Marayti |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | In eukaryotic cells, the shortening and removal of the poly(A) tail (deadenylation) of cytoplasmic mRNA is a key event in regulated mRNA degradation. A major enzyme involved in deadenylation is the Ccr4-Not deadenylase complex, which can be recruited to its target mRNA by RNA-binding proteins or the miRNA repression complex. In addition to six non-catalytic components, the complex contains two enzymatic subunits with ribonuclease activity: Ccr4 and Caf1 (Pop2). In vertebrates, each deadenylase subunit is encoded by two paralogues: Caf1, which can interact with the anti-proliferative protein BTG2, is encoded by CNOT7 and CNOT8, while Ccr4 is encoded by the highly similar genes CNOT6 and CNOT6L. Currently, it is unclear whether the catalytic subunits work cooperatively, or whether the nuclease components have unique roles in deadenylation. We therefore developed a method to express and purify a minimal human BTG2-Caf1-Ccr4 nuclease sub-complex from bacterial cells. By using chemical inhibition and well-characterised inactivating amino acid substitutions, we demonstrate that the enzyme activities of Caf1 and Ccr4 are both required for deadenylation in vitro. These results indicate that Caf1 and Ccr4 cooperate in mRNA deadenylation and suggest that the enzyme activities of Caf1 and Ccr4 are regulated via allosteric interactions within the nuclease module. |
| first_indexed | 2025-11-14T19:04:10Z |
| format | Article |
| id | nottingham-28955 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:04:10Z |
| publishDate | 2015 |
| publisher | Portland Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-289552020-05-04T17:10:28Z https://eprints.nottingham.ac.uk/28955/ The enzyme activities of Caf1 and Ccr4 are both required for deadenylation by the human Ccr4-Not nuclease module Maryati, Marayti Airhihen, Blessing Winkler, G. Sebastiaan In eukaryotic cells, the shortening and removal of the poly(A) tail (deadenylation) of cytoplasmic mRNA is a key event in regulated mRNA degradation. A major enzyme involved in deadenylation is the Ccr4-Not deadenylase complex, which can be recruited to its target mRNA by RNA-binding proteins or the miRNA repression complex. In addition to six non-catalytic components, the complex contains two enzymatic subunits with ribonuclease activity: Ccr4 and Caf1 (Pop2). In vertebrates, each deadenylase subunit is encoded by two paralogues: Caf1, which can interact with the anti-proliferative protein BTG2, is encoded by CNOT7 and CNOT8, while Ccr4 is encoded by the highly similar genes CNOT6 and CNOT6L. Currently, it is unclear whether the catalytic subunits work cooperatively, or whether the nuclease components have unique roles in deadenylation. We therefore developed a method to express and purify a minimal human BTG2-Caf1-Ccr4 nuclease sub-complex from bacterial cells. By using chemical inhibition and well-characterised inactivating amino acid substitutions, we demonstrate that the enzyme activities of Caf1 and Ccr4 are both required for deadenylation in vitro. These results indicate that Caf1 and Ccr4 cooperate in mRNA deadenylation and suggest that the enzyme activities of Caf1 and Ccr4 are regulated via allosteric interactions within the nuclease module. Portland Press 2015-06-19 Article PeerReviewed Maryati, Marayti, Airhihen, Blessing and Winkler, G. Sebastiaan (2015) The enzyme activities of Caf1 and Ccr4 are both required for deadenylation by the human Ccr4-Not nuclease module. Biochemical Journal, 469 (1). pp. 169-176. ISSN 0264-6021 Ccr4-Not; ribonuclease; poly(A); deadenylase; mRNA decay; post-transcriptional gene regulation http://www.biochemj.org/content/469/1/169 doi:10.1042/BJ20150304 doi:10.1042/BJ20150304 |
| spellingShingle | Ccr4-Not; ribonuclease; poly(A); deadenylase; mRNA decay; post-transcriptional gene regulation Maryati, Marayti Airhihen, Blessing Winkler, G. Sebastiaan The enzyme activities of Caf1 and Ccr4 are both required for deadenylation by the human Ccr4-Not nuclease module |
| title | The enzyme activities of Caf1 and Ccr4 are both required for deadenylation by the human Ccr4-Not nuclease module |
| title_full | The enzyme activities of Caf1 and Ccr4 are both required for deadenylation by the human Ccr4-Not nuclease module |
| title_fullStr | The enzyme activities of Caf1 and Ccr4 are both required for deadenylation by the human Ccr4-Not nuclease module |
| title_full_unstemmed | The enzyme activities of Caf1 and Ccr4 are both required for deadenylation by the human Ccr4-Not nuclease module |
| title_short | The enzyme activities of Caf1 and Ccr4 are both required for deadenylation by the human Ccr4-Not nuclease module |
| title_sort | enzyme activities of caf1 and ccr4 are both required for deadenylation by the human ccr4-not nuclease module |
| topic | Ccr4-Not; ribonuclease; poly(A); deadenylase; mRNA decay; post-transcriptional gene regulation |
| url | https://eprints.nottingham.ac.uk/28955/ https://eprints.nottingham.ac.uk/28955/ https://eprints.nottingham.ac.uk/28955/ |