Strategies to overcome photobleaching in algorithm based adaptive optics for nonlinear in-vivo imaging

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Strategies to overcome photobleaching in algorithm based adaptive optics for nonlinear in-vivo imaging

We have developed a nonlinear adaptive optics microscope utilizing a deformable membrane mirror (DMM) and demonstrated its use in compensating for system- and sample-induced aberrations. The optimum shape of the DMM was determined with a random search algorithm optimizing on either two photon fluore...

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Bibliographic Details
Main Authors:	Mullenbroich, M. Caroline, McGhee, Ewan J., Wright, Amanda J., Anderson, Kurt I., Mathieson, Keith
Format:	Article
Published:	Society of Photo-optical Instrumentation Engineers (SPIE) 2014
Subjects:	adaptive optics; aberrations; fluorescence; microscopy; photobleaching
Online Access:	https://eprints.nottingham.ac.uk/2820/

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