Strategies to overcome photobleaching in algorithm based adaptive optics for nonlinear in-vivo imaging
We have developed a nonlinear adaptive optics microscope utilizing a deformable membrane mirror (DMM) and demonstrated its use in compensating for system- and sample-induced aberrations. The optimum shape of the DMM was determined with a random search algorithm optimizing on either two photon fluore...
| Main Authors: | , , , , |
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| Format: | Article |
| Published: |
Society of Photo-optical Instrumentation Engineers (SPIE)
2014
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| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/2820/ |