5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development
5-hydroxymethyl-cytosine (5-hmc) is a cytosine modification that is relatively abundant in mammalian pre-implantation embryos and embryonic stem cells (Esc) derived from mammalian blastocysts. Recent observations imply that both 5-hmc and Tet1/2/3 proteins, catalyzing the conversion of 5-methyl-...
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| Format: | Article |
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Landes Bioscience
2012
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| Online Access: | https://eprints.nottingham.ac.uk/2698/ |
| _version_ | 1848790852720132096 |
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| author | Almeida, Rimple D. Loose, Matthew Sottile, Virginie Matsa, Elena Denning, Chris Young, Lorraine Johnson, Andrew D. Gering, Martin Ruzov, Alexey |
| author_facet | Almeida, Rimple D. Loose, Matthew Sottile, Virginie Matsa, Elena Denning, Chris Young, Lorraine Johnson, Andrew D. Gering, Martin Ruzov, Alexey |
| author_sort | Almeida, Rimple D. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | 5-hydroxymethyl-cytosine (5-hmc) is a cytosine modification that is relatively abundant in mammalian pre-implantation
embryos and embryonic stem cells (Esc) derived from mammalian blastocysts. Recent observations imply that both
5-hmc and Tet1/2/3 proteins, catalyzing the conversion of 5-methyl-cytosine to 5-hmc, may play an important role in
self renewal and differentiation of Escs. here we assessed the distribution of 5-hmc in zebrafish and chick embryos
and found that, unlike in mammals, 5-hmc is immunochemically undetectable in these systems before the onset of
organogenesis. In addition, Tet1/2/3 transcripts are either low or undetectable at corresponding stages of zebrafish
development. however, 5-hmc is enriched in later zebrafish and chick embryos and exhibits tissue-specific distribution in adult zebrafish. Our findings show that 5-hmc enrichment of non-committed cells is not a universal feature of vertebrate development and give insights both into evolution of embryonic pluripotency and the potential role of 5-hmc in its regulation. |
| first_indexed | 2025-11-14T18:19:12Z |
| format | Article |
| id | nottingham-2698 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:19:12Z |
| publishDate | 2012 |
| publisher | Landes Bioscience |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-26982020-05-04T20:21:41Z https://eprints.nottingham.ac.uk/2698/ 5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development Almeida, Rimple D. Loose, Matthew Sottile, Virginie Matsa, Elena Denning, Chris Young, Lorraine Johnson, Andrew D. Gering, Martin Ruzov, Alexey 5-hydroxymethyl-cytosine (5-hmc) is a cytosine modification that is relatively abundant in mammalian pre-implantation embryos and embryonic stem cells (Esc) derived from mammalian blastocysts. Recent observations imply that both 5-hmc and Tet1/2/3 proteins, catalyzing the conversion of 5-methyl-cytosine to 5-hmc, may play an important role in self renewal and differentiation of Escs. here we assessed the distribution of 5-hmc in zebrafish and chick embryos and found that, unlike in mammals, 5-hmc is immunochemically undetectable in these systems before the onset of organogenesis. In addition, Tet1/2/3 transcripts are either low or undetectable at corresponding stages of zebrafish development. however, 5-hmc is enriched in later zebrafish and chick embryos and exhibits tissue-specific distribution in adult zebrafish. Our findings show that 5-hmc enrichment of non-committed cells is not a universal feature of vertebrate development and give insights both into evolution of embryonic pluripotency and the potential role of 5-hmc in its regulation. Landes Bioscience 2012-04 Article PeerReviewed Almeida, Rimple D., Loose, Matthew, Sottile, Virginie, Matsa, Elena, Denning, Chris, Young, Lorraine, Johnson, Andrew D., Gering, Martin and Ruzov, Alexey (2012) 5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development. Epigenetics, 7 (4). pp. 383-389. ISSN 1559-2294 https://www.landesbioscience.com/journals/epigenetics/article/19375/ doi:10.4161/epi.19375 doi:10.4161/epi.19375 |
| spellingShingle | Almeida, Rimple D. Loose, Matthew Sottile, Virginie Matsa, Elena Denning, Chris Young, Lorraine Johnson, Andrew D. Gering, Martin Ruzov, Alexey 5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development |
| title | 5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development |
| title_full | 5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development |
| title_fullStr | 5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development |
| title_full_unstemmed | 5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development |
| title_short | 5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development |
| title_sort | 5-hydroxymethyl-cytosine enrichment of non-committed cells is not a universal feature of vertebrate development |
| url | https://eprints.nottingham.ac.uk/2698/ https://eprints.nottingham.ac.uk/2698/ https://eprints.nottingham.ac.uk/2698/ |