Adenosine-A3 receptors in neutrophil microdomains promote the formation of bacteria-tethering cytonemes
The A3‐adenosine receptor (A3AR) has recently emerged as a key regulator of neutrophil behaviour. Using a fluorescent A3AR ligand, we show that A3ARs aggregate in highly polarized immunomodulatory microdomains on human neutrophil membranes. In addition to regulating chemotaxis, A3ARs promote the for...
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| Format: | Article |
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Nature Publishing Group
2013
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| Online Access: | https://eprints.nottingham.ac.uk/2675/ |
| _version_ | 1848790846617419776 |
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| author | Corriden, Ross Self, Tim Akong-Moore, Kathryn Nizet, Victor Kellam, Barrie Briddon, Stephen J. Hill, Stephen J. |
| author_facet | Corriden, Ross Self, Tim Akong-Moore, Kathryn Nizet, Victor Kellam, Barrie Briddon, Stephen J. Hill, Stephen J. |
| author_sort | Corriden, Ross |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The A3‐adenosine receptor (A3AR) has recently emerged as a key regulator of neutrophil behaviour. Using a fluorescent A3AR ligand, we show that A3ARs aggregate in highly polarized immunomodulatory microdomains on human neutrophil membranes. In addition to regulating chemotaxis, A3ARs promote the formation of filipodia‐like projections (cytonemes) that can extend up to 100 μm to tether and ‘reel in’ pathogens. Exposure to bacteria or an A3AR agonist stimulates the formation of these projections and bacterial phagocytosis, whereas an A3AR‐selective antagonist inhibits cytoneme formation. Our results shed new light on the behaviour of neutrophils and identify the A3AR as a potential target for modulating their function. |
| first_indexed | 2025-11-14T18:19:06Z |
| format | Article |
| id | nottingham-2675 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:19:06Z |
| publishDate | 2013 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-26752020-05-04T16:38:09Z https://eprints.nottingham.ac.uk/2675/ Adenosine-A3 receptors in neutrophil microdomains promote the formation of bacteria-tethering cytonemes Corriden, Ross Self, Tim Akong-Moore, Kathryn Nizet, Victor Kellam, Barrie Briddon, Stephen J. Hill, Stephen J. The A3‐adenosine receptor (A3AR) has recently emerged as a key regulator of neutrophil behaviour. Using a fluorescent A3AR ligand, we show that A3ARs aggregate in highly polarized immunomodulatory microdomains on human neutrophil membranes. In addition to regulating chemotaxis, A3ARs promote the formation of filipodia‐like projections (cytonemes) that can extend up to 100 μm to tether and ‘reel in’ pathogens. Exposure to bacteria or an A3AR agonist stimulates the formation of these projections and bacterial phagocytosis, whereas an A3AR‐selective antagonist inhibits cytoneme formation. Our results shed new light on the behaviour of neutrophils and identify the A3AR as a potential target for modulating their function. Nature Publishing Group 2013-07-02 Article PeerReviewed Corriden, Ross, Self, Tim, Akong-Moore, Kathryn, Nizet, Victor, Kellam, Barrie, Briddon, Stephen J. and Hill, Stephen J. (2013) Adenosine-A3 receptors in neutrophil microdomains promote the formation of bacteria-tethering cytonemes. EMBO reports, 14 (8). pp. 726-732. ISSN 1469-221X http://embor.embopress.org/content/14/8/726 doi:10.1038/embor.2013.89 doi:10.1038/embor.2013.89 |
| spellingShingle | Corriden, Ross Self, Tim Akong-Moore, Kathryn Nizet, Victor Kellam, Barrie Briddon, Stephen J. Hill, Stephen J. Adenosine-A3 receptors in neutrophil microdomains promote the formation of bacteria-tethering cytonemes |
| title | Adenosine-A3 receptors in neutrophil microdomains
promote the formation of bacteria-tethering cytonemes |
| title_full | Adenosine-A3 receptors in neutrophil microdomains
promote the formation of bacteria-tethering cytonemes |
| title_fullStr | Adenosine-A3 receptors in neutrophil microdomains
promote the formation of bacteria-tethering cytonemes |
| title_full_unstemmed | Adenosine-A3 receptors in neutrophil microdomains
promote the formation of bacteria-tethering cytonemes |
| title_short | Adenosine-A3 receptors in neutrophil microdomains
promote the formation of bacteria-tethering cytonemes |
| title_sort | adenosine-a3 receptors in neutrophil microdomains
promote the formation of bacteria-tethering cytonemes |
| url | https://eprints.nottingham.ac.uk/2675/ https://eprints.nottingham.ac.uk/2675/ https://eprints.nottingham.ac.uk/2675/ |