Native-state stability determines the extent of degradation relative to secretion of protein variants from Pichia pastoris

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Native-state stability determines the extent of degradation relative to secretion of protein variants from Pichia pastoris

We have investigated the relationship between the stability and secreted yield of a series of mutational variants of human lysozyme (HuL) in Pichia pastoris. We show that genes directly involved in the unfolded protein response (UPR), ER-associated degradation (ERAD) and ER-phagy are transcriptional...

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Bibliographic Details
Main Authors:	Whyteside, Graham, Alcocer, Marcos J.C., Kumita, Janet R., Dobson, Christopher M., Lazarou, Maria, Pleass, Richard J., Archer, David B.
Format:	Article
Published:	Public Library of Science 2011
Online Access:	https://eprints.nottingham.ac.uk/2585/

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