Native-state stability determines the extent of degradation relative to secretion of protein variants from Pichia pastoris
We have investigated the relationship between the stability and secreted yield of a series of mutational variants of human lysozyme (HuL) in Pichia pastoris. We show that genes directly involved in the unfolded protein response (UPR), ER-associated degradation (ERAD) and ER-phagy are transcriptional...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Published: |
Public Library of Science
2011
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| Online Access: | https://eprints.nottingham.ac.uk/2585/ |