Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles

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Expanding the repertoire of gene tools for precise manipulation of the Clostridium difficile genome: allelic exchange using pyrE alleles

Sophisticated genetic tools to modify essential biological processes at the molecular level are pivotal in elucidating the molecular pathogenesis of Clostridium difficile, a major cause of healthcare associated disease. Here we have developed an efficient procedure for making precise alterations to...

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Bibliographic Details
Main Authors:	Ng, Yen K., Ehsaan, Muhammad, Philip, Sheryl, Collery, Mark M., Janoir, Clare, Collignon, Anne, Cartman, Stephen T., Minton, Nigel P.
Format:	Article
Published:	Public Library of Science 2013
Online Access:	https://eprints.nottingham.ac.uk/2536/

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